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ZC3H12D Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZC3H12D Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ZC3H12D antibodies →
$504.00each
Catalog Number: LS-G102773-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZC3H12D (NM_207360), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DJ281H8.1 | MCPIP4 | p34 | ZC3H12D | Tumor suppressor TFL | C6orf95 | MCP-induced protein 4 | Probable ribonuclease ZC3H12D | TFL
Origin Species
Human
SwissProt
A2A288

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
58.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEHPSKMEFF QKLGYDREDV LRVLGKLGEG ALVNDVLQEL IRTGSRPGAL EHPAAPRLVP
61RGSCGVPDSA QRGPGTALEE DFRTLASSLR PIVIDGSNVA MSHGNKETFS CRGIKLAVDW
121FRDRGHTYIK VFVPSWRKDP PRADTPIREQ HVLAELERQA VLVYTPSRKV HGKRLVCYDD
181RYIVKVAYEQ DGVIVSNDNY RDLQSENPEW KWFIEQRLLM FSFVNDRFMP PDDPLGRHGP
241SLSNFLSRKP KPPEPSWQHC PYGKKCTYGI KCKFYHPERP HHAQLAVADE LRAKTGARPG
301AGAEEQRPPR APGGSAGARA APREPFAHSL PPARGSPDLA ALRGSFSRLA FSDDLGPLGP
361PLPVPACSLT PRLGGPDWVS AGGRVPGPLS LPSPESQFSP GDLPPPPGLQ LQPRGEHRPR
421DLHGDLLSPR RPPDDPWARP PRSDRFPGRS VWAEPAWGDG ATGGLSVYAT EDDEGDARAR
481ARIALYSVFP RDQVDRVMAA FPELSDLARL ILLVQRCQSA GAPLGKP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

527 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.