LSBio
ZC3H12B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZC3H12B Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ZC3H12B antibodies →
$725.00each
Catalog Number: LS-G121718-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZC3H12B (NM_001010888), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CXorf32 | MCPIP2 | MCP-induced protein 2 | ZC3H12B | Probable ribonuclease ZC3H12B
Origin Species
Human
SwissProt
Q5HYM0

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
94.21 kDa
Protein Type
Lysate
Amino Acid Sequence
1MTATAEVETP KMEKSASKEE KQQPKQDSTE QGNADSEEWM SSESDPEQIS LKSSDNSKSC
61QPRDGQLKKK EMHSKPHRQL CRSPCLDRPS FSQSSILQDG KLDLEKEYQA KMEFALKLGY
121AEEQIQSVLN KLGPESLIND VLAELVRLGN KGDSEGQINL SLLVPRGPSS REIASPELSL
181EDEIDNSDNL RPVVIDGSNV AMSHGNKEEF SCRGIQLAVD WFLDKGHKDI TVFVPAWRKE
241QSRPDAPITD QDILRKLEKE KILVFTPSRR VQGRRVVCYD DRFIVKLAFD SDGIIVSNDN
301YRDLQVEKPE WKKFIEERLL MYSFVNDKFM PPDDPLGRHG PSLENFLRKR PIVPEHKKQP
361CPYGKKCTYG HKCKYYHPER ANQPQRSVAD ELRISAKLST VKTMSEGTLA KCGTGMSSAK
421GEITSEVKRV APKRQSDPSI RSVAMEPEEW LSIARKPEAS SVPSLVTALS VPTIPPPKSH
481AVGALNTRSA SSPVPGSSHF PHQKASLEHM ASMQYPPILV TNSHGTPISY AEQYPKFESM
541GDHGYYSMLG DFSKLNINSM HNREYYMAEV DRGVYARNPN LCSDSRVSHT RNDNYSSYNN
601VYLAVADTHP EGNLKLHRSA SQNRLQPFPH GYHEALTRVQ SYGPEDSKQG PHKQSVPHLA
661LHAQHPSTGT RSSCPADYPM PPNIHPGATP QPGRALVMTR MDSISDSRLY ESNPVRQRRP
721PLCREQHASW DPLPCTTDSY GYHSYPLSNS LMQPCYEPVM VRSVPEKMEQ LWRNPWVGMC
781NDSREHMIPE HQYQTYKNLC NIFPSNIVLA VMEKNPHTAD AQQLAALIVA KLRAAR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

836 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.