LSBio
ZBTB7C Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZBTB7C Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ZBTB7C antibodies →
$725.00each
Catalog Number: LS-G120586-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZBTB7C (NM_001039360), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
APM-1 | APM1 | ZBTB7C | Zinc finger protein 857C | ZBTB36 | ZNF857C
Origin Species
Human
NCBI GenBank
NM_001039360
SwissProt
A1YPR0

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
68.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MANDIDELIG IPFPNHSSEV LCSLNEQRHD GLLCDVLLVV QEQEYRTHRS VLAACSKYFK
61KLFTAGTLAS QPYVYEIDFV QPEALAAILE FAYTSTLTIT AGNVKHILNA ARMLEIQCIV
121NVCLEIMEPG GDGGEEDDKE DDDDDEDDDD EEDEEEEEEE EEDDDDDTED FADQENLPDP
181QDISCHQSPS KTDHLTEKAY SDTPRDFPDS FQAGSPGHLG VIRDFSIESL LRENLYPKAN
241IPDRRPSLSP FAPDFFPHLW PGDFGAFAQL PEQPMDSGPL DLVIKNRKIK EEEKEELPPP
301PPPPFPNDFF KDMFPDLPGG PLGPIKAEND YGAYLNFLSA THLGGLFPPW PLVEERKLKP
361KASQQCPICH KVIMGAGKLP RHMRTHTGEK PYMCTICEVR FTRQDKLKIH MRKHTGERPY
421LCIHCNAKFV HNYDLKNHMR IHTGVRPYQC EFCYKSFTRS DHLHRHIKRQ SCRMARPRRG
481RKPAAWRAAS LLFGPGGPAP DKAAFVMPPA LGEVGGHLGG AAVCLPGPSP AKHFLAAPKG
541ALSLQELERQ FEETQMKLFG RAQLEAERNA GGLLAFALAE NVAAARPYFP LPDPWAAGLA
601GLPGLAGLNH VASMSEANN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

619 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.