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ZBTB5 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZBTB5 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G113569-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZBTB5 (NM_014872), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ZBTB5 | KIAA0354
Origin Species
Human
SwissProt
O15062

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
74.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDFPGHFEQI FQQLNYQRLH GQLCDCVIVV GNRHFKAHRS VLAACSTHFR ALFSVAEGDQ
61TMNMIQLDSE VVTAEAFAAL IDMMYTSTLM LGESNVMDVL LAASHLHLNS VVKACKHYLT
121TRTLPMSPPS ERVQEQSARM QRSFMLQQLG LSIVSSALNS SQNGEEQPAP MSSSMRSNLD
181QRTPFPMRRL HKRKQSAEER ARQRLRPSID ESAISDVTPE NGPSGVHSRE EFFSPDSLKI
241VDNPKADGMT DNQEDSAIMF DQSFGTQEDA QVPSQSDNSA GNMAQLSMAS RATQVETSFD
301QEAAPEKSSF QCENPEVGLG EKEHMRVVVK SEPLSSPEPQ DEVSDVTSQA EGSESVEVEG
361VVVSAEKIDL SPESSDRSFS DPQSSTDRVG DIHILEVTNN LEHKSTFSIS NFLNKSRGNN
421FTANQNNDDN IPNTTSDCRL ESEAPYLLSP EAGPAGGPSS APGSHVENPF SEPADSHFVR
481PMQEVMGLPC VQTSGYQGGE QFGMDFSRSG LGLHSSFSRV MIGSPRGGAS NFPYYRRIAP
541KMPVVTSVRS SQIPENSTSS QLMMNGATSS FENGHPSQPG PPQLTRASAD VLSKCKKALS
601EHNVLVVEGA RKYACKICCK TFLTLTDCKK HIRVHTGEKP YACLKCGKRF SQSSHLYKHS
661KTTCLRWQSS NLPSTLL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

677 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.