Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ZBTB33 / Kaiso Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G115086-100
Catalog Number: LS-G115086
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Kaiso(ZBTB33) (NM_006777), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Kaiso | ZBTB33 | WUGSC:H_DJ525N14.1 | ZNF-kaiso | Kaiso transcription factor | ZNF348
- Origin Species
- Human
- NCBI GenBank
- NM_006777
- SwissProt
- Q86T24
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 74.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MESRKLISAT DIQYSGSLLN SLNEQRGHGL FCDVTVIVED RKFRAHKNIL SASSTYFHQL61FSVAGQVVEL SFIRAEIFAE ILNYIYSSKI VRVRSDLLDE LIKSGQLLGV KFIAELGVPL121SQVKSISGTA QDGNTEPLPP DSGDKNLVIQ KSKDEAQDNG ATIMPIITES FSLSAEDYEM181KKIIVTDSDD DDDDDVIFCS EILPTKETLP SNNTVAQVQS NPGPVAISDV APSASNNSPP241LTNITPTQKL PTPVNQATLS QTQGSEKLLV SSAPTHLTPN IILLNQTPLS TPPNVSSSLP301NHMPSSINLL VQNQQTPNSA ILTGNKANEE EEEEIIDDDD DTISSSPDSA VSNTSLVPQA361DTSQNTSFDG SLIQKMQIPT LLQEPLSNSL KISDIITRNT NDPGVGSKHL MEGQKIITLD421TATEIEGLST GCKVYANIGE DTYDIVIPVK DDPDEGEARL ENEIPKTSGS EMANKRMKVK481HDDHYELIVD GRVYYICIVC KRSYVCLTSL RRHFNIHSWE KKYPCRYCEK VFPLAEYRTK541HEIHHTGERR YQCLACGKSF INYQFMSSHI KSVHSQDPSG DSKLYRLHPC RSLQIRQYAY601LSDRSSTIPA MKDDGIGYKV DTGKEPPVGT TTSTQNKPMT WEDIFIQQEN DSIFKQNVTD661GSTEFEFIIP ESYTRTRPLEQKLISEEDLAANDILDYKDDDDKV
673 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.