Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ZBTB32 / TZFP Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G113991-100
Catalog Number: LS-G113991
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TZFP(ZBTB32) (NM_014383), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- FAXF | FANCC-interacting protein | FAZF | Rog | Testis zinc finger protein | ZNF538 | TZFP | Zinc finger protein 538 | Repressor of GATA | Testis zinc finger protein 91 | ZBTB32
- Origin Species
- Human
- NCBI GenBank
- NM_014383
- SwissProt
- Q9Y2Y4
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 53.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSLPPIRLPS PYGSDRLVQL AARLRPALCD TLITVGSQEF PAHSLVLAGV SQQLGRRGQW61ALGEGISPST FAQLLNFVYG ESVELQPGEL RPLQEAARAL GVQSLEEACW RARGDRAKKP121DPGLKKHQEE PEKPSRNPER ELGDPGEKQK PEQVSRTGGR EQEMLHKHSP PRGRPEMAGA181TQEAQQEQTR SKEKRLQAPV GQRGADGKHG VLTWLRENPG GSEESLRKLP GPLPPAGSLQ241TSVTPRPSWA EAPWLVGGQP ALWSILLMPP RYGIPFYHST PTTGAWQEVW REQRIPLSLN301APKGLWSQNQ LASSSPTPGS LPQGPAQLSP GEMEESDQGH TGALATCAGH EDKAGCPPRP361HPPPAPPARS RPYACSVCGK RFSLKHQMET HYRVHTGEKP FSCSLCPQRS RDFSAMTKHL421RTHGAAPYRC SLCGAGCPSL ASMQAHMRGH SPSQLPPGWT IRSTFLYSSS RPSRPSTSPC481CPSSSTTTRTRPLEQKLISEEDLAANDILDYKDDDDKV
487 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.