Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ZBTB24 / BIF1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G113673-100
Catalog Number: LS-G113673
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZBT24(ZBTB24) (NM_014797), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- PATZ2 | KIAA0441 | BIF1 | Zinc finger protein 450 | ZNF450 | ICF2 | ZBTB24
- Origin Species
- Human
- NCBI GenBank
- AY210403
- SwissProt
- Q80X44
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 78.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAETSPEPSG QLVVHSDAHS DTVLASFEDQ RKKGFLCDIT LIVENVHFRA HKALLAASSE61YFSMMFAEEG EIGQSIYMLE GMVADTFGIL LEFIYTGYLH ASEKSTEQIL ATAQFLKVYD121LVKAYTDFQN NHSSPKPTTL NTAGAPVVVI SNKKNDPPKR KRGRPKKVNT LQEEKSELAA181EEEIQLRVNN SVQNRQNFVV KGDSGVLNEQ IAAKEKEESE PTCEPSREEE MPVEKDENYD241PKTEDGQASQ SRYSKRRIWR SVKLKDYKLV GDQEDHGSAK RICGRRKRPG GPEARCKDCG301KVFKYNHFLA IHQRSHTGER PFKCNECGKG FAQKHSLQVH TRMHTGERPY TCTVCSKALT361TKHSLLEHMS LHSGQKSFTC DQCGKYFSQN RQLKSHYRVH TGHSLPECKD CHRKFMDVSQ421LKKHLRTHTG EKPFTCEICG KSFTAKSSLQ THIRIHRGEK PYSCGICGKS FSDSSAKRRH481CILHTGKKPF SCPECNLQFA RLDNLKAHLK IHSKEKHASD ASSISGSSNT EEVRNILQLQ541PYQLSTSGEQ EIQLLVTDSV HNINFMPGPS QGISIVTAES SQNMTADQAA NLTLLTQQPE601QLQNLILSAQ QEQTEHIQSL NMIESQMGPS QTEPVHVITL SKETLEHLHA HQEQTEELHL661ATSTSDPAQH LQLTQEPGPP PPTHHVPQPT PLGQEQSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
697 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.