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ZBTB22 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZBTB22 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ZBTB22 antibodies →
$504.00each
Catalog Number: LS-G126654-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZBTB22 (NM_001145338), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BING1 | Fru | Fruitless | ZNF297 | ZBTB22 | ZBTB22A | Zinc finger protein 297 | ZNF297A | Protein BING1
Origin Species
Human
NCBI GenBank
NM_005453
SwissProt
O15209

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
65.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEPSPLSPSG AALPLPLSLA PPPLPLPAAA VVHVSFPEVT SALLESLNQQ RLQGQLCDVS
61IRVQGREFRA HRAVLAASSP YFHDQVLLKG MTSISLPSVM DPGAFETVLA SAYTGRLSMA
121AADIVNFLTV GSVLQMWHIV DKCTELLREG RASATTTITT AAATSVTVPG AGVPSGSGGT
181VAPATMGSAR SHASSRASEN QSPSSSNYFS PRESTDFSSS SQEAFAASAV GSGERRGGGP
241VFPAPVVGSG GATSGKLLLE ADELCDDGGD GRGAVVPGAG LRRPTYTPPS IMPQKHWVYV
301KRGGNCPAPT PLVPQDPDLE EEEEEEDLVL TCEDDEDEEL GGSSRVPVGG GPEATLSISD
361VRTLSEPPDK GEEQVNFCES SNDFGPYEGG GPVAGLDDSG GPTPSSYAPS HPPRPLLPLD
421MQGNQILVFP SSSSSSSSQA PGQPPGNQAE HGAVTVGGTS VGSLGVPGSV GGVPGGTGSG
481DGNKIFLCHC GKAFSHKSMR DRHVNMHLNL RPFDCPVCNK KFKMKHHLTE HMKTHTGLKP
541YECGVCAKKF MWRDSFMRHR GHCERRHRLG GVGAVPGPGT PTGPSLPSKR ESPGVGGGSG
601DEASAATPPS SRRVWSPPRV HKVEMGFGGG GGAN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

634 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.