LSBio
ZBTB20 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZBTB20 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ZBTB20 antibodies →
$725.00each
Catalog Number: LS-G128760-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZBTB20 (NM_001164347), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DPZF | ODA-8S | ZBTB20 | HOF | Zinc finger protein 288 | ZNF288
Origin Species
Human
NCBI GenBank
NM_015642

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
73.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MTERIHSINL HNFSNSVLET LNEQRNRGHF CDVTVRIHGS MLRAHRCVLA AGSPFFQDKL
61LLGYSDIEIP SVVSVQSVQK LIDFMYSGVL RVSQSEALQI LTAASILQIK TVIDECTRIV
121SQNVGDVFPG IQDSGQDTPR GTPESGTSGQ SSDTESGYLQ SHPQHSVDRI YSALYACSMQ
181NGSGERSFYS GAVVSHHETA LGLPRDHHME DPSWITRIHE RSQQMERYLS TTPETTHCRK
241QPRPVRIQTL VGNIHIKQEM EDDYDYYGQQ RVQILERNES EECTEDTDQA EGTESEPKGE
301SFDSGVSSSI GTEPDSVEQQ FGPGAARDSQ AEPTQPEQAA EAPAEGGPQT NQLETGASSP
361ERSNEVEMDS TVITVSNSSD KSVLQQPSVN TSIGQPLPST QLYLRQTETL TSNLRMPLTL
421TSNTQVIGTA GNTYLPALFT TQPAGSGPKP FLFSLPQPLA GQQTQFVTVF QPGLSTFTAQ
481LPAPQPLASS AGHSTASGQG EKKPYECTLC NKTFTAKQNY VKHMFVHTGE KPHQCSICWR
541SFSLKDYLIK HMVTHTGVRA YQCSICNKRF TQKSSLNVHM RLHRGEKSYE CYICKKKFSH
601KTLLERHVAL HSASNGTPPA GTPPGARAGP PGVVACTEGT TYVCSVCPAK FDQIEQFNDH
661MRMHVSDG
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

668 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.