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ZBTB2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZBTB2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G110915-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZBTB2 (NM_020861), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BA351K16.2 | KIAA1483 | ZBTB2 | ZNF437
Origin Species
Human
SwissProt
Q8N680

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
57.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDLANHGLIL LQQLNAQREF GFLCDCTVAI GDVYFKAHKS VLASFSNYFK MLFVHQTSEC
61VRLKPTDIQP DIFSYLLHLM YTGKMAPQLI DPVRLEQGIK FLHAYPLIQE ASLASQGAFS
121HPDQVFPLAS SLYGIQIADH QLRQATKIAS APEKLGRDPR PQTSRISQEQ VPEASQLSQL
181TSNLAQVNRT NMTPSDPLQT SLSPELVSTP VPPPPPGEET NLEASSSDEQ PASLTIAHVK
241PSIMKRNGSF PKYYACHLCG RRFTLRSSLR EHLQIHTGVP FTSSQQGESR VPLTLCSNAA
301DLGKDAMEVP EAGMISDSEL QHISDSPIID GQQQSETPPP SDIADIDNLE QADQEREVKR
361RKYECTICGR KFIQKSHWRE HMYIHTGKPF KCSTCDKSFC RANQAARHVC LNQSIDTYTM
421VDKQTLELCT FEEGSQMDNM LVQTNKPYKC NLCDKTFSTP NEVVKHSCQN QNSDVFALDE
481GRSILLGSGD SEVTEPDHPV LASIKKEQET VLLD
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

514 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.