Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ZBTB17 / MIZ-1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G100050-100
Catalog Number: LS-G100050
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Miz1(ZBTB17) (NM_003443), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- MIZ-1 | MIZ1 | Zinc finger protein 151 | ZNF60 | ZBTB17 | PHZ-67 | Zinc finger protein 60 | ZNF151
- Origin Species
- Human
- NCBI GenBank
- NM_003443
- SwissProt
- Q13105
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 87.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDFPQHSQHV LEQLNQQRQL GLLCDCTFVV DGVHFKAHKA VLAACSEYFK MLFVDQKDVV61HLDISNAAGL GQVLEFMYTA KLSLSPENVD DVLAVATFLQ MQDIITACHA LKSLAEPATS121PGGNAEALAT EGGDKRAKEE KVATSTLSRL EQAGRSTPIG PSRDLKEERG GQAQSAASGA181EQTEKADAPR EPPPVELKPD PTSGMAAAEA EAALSESSEQ EMEVEPARKG EEEQKEQEEQ241EEEGAGPAEV KEEGSQLENG EAPEENENEE SAGTDSGQEL GSEARGLRSG TYGDRTESKA301YGSVIHKCED CGKEFTHTGN FKRHIRIHTG EKPFSCRECS KAFSDPAACK AHEKTHSPLK361PYGCEECGKS YRLISLLNLH KKRHSGEARY RCEDCGKLFT TSGNLKRHQL VHSGEKPYQC421DYCGRSFSDP TSKMRHLETH DTDKEHKCPH CDKKFNQVGN LKAHLKIHIA DGPLKCRECG481KQFTTSGNLK RHLRIHSGEK PYVCIHCQRQ FADPGALQRH VRIHTGEKPC QCVMCGKAFT541QASSLIAHVR QHTGEKPYVC ERCGKRFVQS SQLANHIRHH DNIRPHKCSV CSKAFVNVGD601LSKHIIIHTG EKPYLCDKCG RGFNRVDNLR SHVKTVHQGK AGIKILEPEE GSEVSVVTVD661DMVTLATEAL AATAVTQLTV VPVGAAVTAD ETEVLKAEIS KAVKQVQEED PNTHILYACD721SCGDKFLDAN SLAQHVRIHT AQALVMFQTD ADFYQQYGPG GTWPAGQVLQ AGELVFRPRD781GAEGQPALAE TSPTAPECPP PAETRTRPLEQKLISEEDLAANDILDYKDDDDKV
803 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.