Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ZBTB12 / G10 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G104309-100
Catalog Number: LS-G104309
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZBTB12 (NM_181842), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Bat9 | D6S59E | G10 | HLA-B-associated transcript 9 | NG35 | Protein G10 | C6orf46 | ZBTB12
- Origin Species
- Human
- SwissProt
- Q9Y330
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 49 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MASGVEVLRF QLPGHEAATL RNMNQLRAEE RFCDVTIVAD SLKFRGHKVI LAACSPFLRD61QFLLNPSSEL QVSLMHSARI VADLLLSCYT GALEFAVRDI VNYLTAASYL QMEHVVEKCR121NALSQFIEPK IGLKEDGVSE ASLVSSISAT KSLLPPARTP KPAPKPPPPP PLPPPLLRPV181KLEFPLDEDL ELKAEEEDED EDEDVSDICI VKVESALEVA HRLKPPGGLG GGLGIGGSVG241GHLGELAQSS VPPSTVAPPQ GVVKACYSLS EDAEGEGLLL IPGGRASVGA TSGLVEAAAV301AMAARGAGGS LGAGGSRGPL PGGFSGGNPL KNIKCTKCPE VFQGVEKLVF HMRAQHFIFM361CPRCGKQFNH SSNLNRHMNV HRGVKSHSCG ICGKCFTQKS TLHDHLNLHS GARPYRCSYC421DVRFAHKPAI RRHLKEQHGK TTAENVLEAS VAEINVLIRTRTRPLEQKLISEEDLAANDILDYKDDDDKV
459 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.