LSBio
ZBTB10 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZBTB10 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G124138-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZBTB10 (NM_001105539), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
RINZF | RINZFC | ZBTB10 | Zinc finger protein RIN ZF | Zinc finger protein RINZF
Origin Species
Human
NCBI GenBank
NM_023929
SwissProt
Q96DT7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
94.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSFSEMNRRT LAFRGGGLVT ASGGGSTNNN AGGEASAWPP QPQPRQPPPP APPALQPPNG
61RGADEEVELE GLEPQDLEAS AGPAAGAAEE AKELLLPQDA GGPTSLGGGA GGPLLAERNR
121RTLAFRGGGG GGLGNNGSSR GRPETSVWPL RHFNGRGPAT VDLELDALEG KELMQDGASL
181SDSTEDEEEG ASLGDGSGAE GGSCSSSRRS GGDGGDEVEG SGVGAGEGET VQHFPLARPK
241SLMQKLQCSF QTSWLKDFPW LRYSKDTGLM SCGWCQKTPA DGGSVDLPPV GHDELSRGTR
301NYKKTLLLRH HVSTEHKLHE ANAQESEIPS EEGYCDFNSR PNENSYCYQL LRQLNEQRKK
361GILCDVSIVV SGKIFKAHKN ILVAGSRFFK TLYCFSNKES PNQNNTTHLD IAAVQGFSVI
421LDFLYSGNLV LTSQNAIEVM TVASYLQMSE VVQTCRNFIK DALNISIKSE APESVVVDYN
481NRKPVNRDGL SSSRDQKIAS FWATRNLTNL ASNVKIENDG CNVDEGQIEN YQMNDSSWVQ
541DGSPEMAENE SEGQTKVFIW NNMGSQGIQE TGKTRRKNQT TKRFIYNIPP NNETNLEDCS
601VMQPPVAYPE ENTLLIKEEP DLDGALLSGP DGDRNVNANL LAEAGTSQDG GDAGTSHDFK
661YGLMPGPSND FKYGLIPGTS NDFKYGLIPG ASNDFKYGLL PESWPKQETW ENGESSLIMN
721KLKCPHCSYV AKYRRTLKRH LLIHTGVRSF SCDICGKLFT RREHVKRHSL VHKKDKKYKC
781MVCKKIFMLA ASVGIRHGSR RYGVCVDCAD KSQPGGQEGV DQGQDTEFPR DEEYEENEVG
841EADEELVDDG EDQNDPSRWD ESGEVCMSLD D
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

871 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.