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ZBTB1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZBTB1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G113583-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZBTB1 (NM_014950), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA0997 | ZBTB1 | ZNF909
Origin Species
Human
NCBI GenBank
AB023214
SwissProt
Q9Y2K1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
73.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAKPSHSSYV LQQLNNQREW GFLCDCCIAI DDIYFQAHKA VLAACSSYFR MFFMNHQHST
61AQLNLSNMKI SAECFDLILQ FMYLGKIMTA PSSFEQFKVA MNYLQLYNVP DCLEDIQDAD
121CSSSKCSSSA SSKQNSKMIF GVRMYEDTVA RNGNEANRWC AEPSSTVNTP HNREADEESL
181QLGNFPEPLF DVCKKSSVSK LSTPKERVSR RFGRSFTCDS CGFGFSCEKL LDEHVLTCTN
241RHLYQNTRSY HRIVDIRDGK DSNIKAEFGE KDSSKTFSAQ TDKYRGDTSQ AADDSASTTG
301SRKSSTVESE IASEEKSRAA ERKRIIIKME PEDIPTDELK DFNIIKVTDK DCNESTDNDE
361LEDEPEEPFY RYYVEEDVSI KKSGRKTLKP RMSVSADERG GLENMRPPNN SSPVQEDAEN
421ASCELCGLTI TEEDLSSHYL AKHIENICAC GKCGQILVKG RQLQEHAQRC GEPQDLTMNG
481LGNTEEKMDL EENPDEQSEI RDMFVEMLDD FRDNHYQINS IQKKQLFKHS ACPFRCPNCG
541QRFETENLVV EHMSSCLDQD MFKSAIMEEN ERDHRRKHFC NLCGKGFYQR CHLREHYTVH
601TKEKQFVCQT CGKQFLRERQ LRLHNDMHKG MASGEIGPSK PVEK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

644 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.