Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ZBED9 / SCAND3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G108083-100
Catalog Number: LS-G108083
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SCAND3(ZBED9) (NM_052923), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DJ1186N24.3 | ZFP38-L | Protein ZNF452 | SCAN domain containing 3 | Zinc finger protein 452 | ZNF305P2 | ZNF452 | KIAA1925 | SCAND3
- Origin Species
- Human
- NCBI GenBank
- AA481935
- SwissProt
- Q6R2W3
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 151.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEAVSRVFPA LAGQAPEEQG EIIKVKVKEE DHTWDQESAL RRNLSYTREL SRQRFRQFCY61QETPGPREAL SQLRELCRQW LNPEIHTKEQ ILELLVLEQF LTILPEELQS WVREHNPESG121EEVVTLLEDL ERELDEPRQQ VSQGTYGQEV SMEEMIPLDS AKESLGTQLQ SMEDRMECES181PEPHPLQDNG SFLWFSMMSQ SMGGDNLSSL DTNEAEIEPE NMREKFFRSL ARLLENKSNN241TKIFSKAKYC QLIKEVKEAK AKAKKESVDY RRLARFDVIL VQGNEKLIEA VNGETDKIRY301YLHSEDLFDI LHNTHLSIGH GGRTRMEKEL QAKYKNITKE VIMLYLTLCK PCQQKNSKLK361KVLTSKSIKE VSSRCQVDLI DMQLNPDGEY RFILHYQDLC TKLTFLRSLK SKRPTEVAHA421LLDIFTIIGA PSVLQSDNGR EFSSQVVSEL SNIWPELKIV HGKSQTCQSQ SSAEQTEDIR481KRIFSWMQTN NSSHWTEFLW FIQMSQNQPY HRSMQQTPCE SAFSSEAKLG LSHSQLTEEL541VASLHTENEL DQADKELENT LRAQYEENIE TGTDSSDIEE NLSVTPKVAE KSPPESRLRF601LSCVVCEKEC TGVNSCISCD GNIHAICGVP SQHGTEGCGR QITCSLCYET STMKRKHDEI661QRSLPVKPSK MLKPSGTPFS PDKVGDWMAK QASLDFFVKK RHAFSEHSSS NKRNVNNRSY721PEEGKTKRVH ASFTRKYDPS YIEFGFVAVI DGEVLKPQCI ICGDVLANEA MKPSKLKRHL781YSKHKEISSQ PKEFFERKSS ELKSQPKQVF NVSHINISAL RASYKVALPV AKSKTPYTIA841ETLVKDCIKE VCLEMLGESA AKKVAQVPLS NDTIARRIQE LANDMEDQLI EQIKLAKYFS901LQLDECRDIA NMIILLVYVR FEHDDDIKEE FFFSASLPTN TTSSELYEAV KNYIVNKCGL961EFKFCVGVCS DGAASMTGKH SEVVTQIKEL APECKTTHCF IHRESLAMKK ISAELNSVLN1021DIVKIVNYIK SNSLNSRLFS LLCDNMEADH KQLLLHAEIR WLSRGKVLSR MFEIRNELLV1081FLQGKKPMWS QLFKDVNWTA RLAYLSDIFS IFNDLNASMQ GKNATYFSMA DKVEGQKQKL1141EAWKNRISTD CYDMFHNLTT IINEVGNDLD IAHLRKVISE HLTNLLECFE FYFPSKEDPR1201IGNLWIQNPF LSSKDNLNLT VTLQDKLLKL ATDEGLKISF ENTASLPSFW IKAKNDYPEL1261AEIALKLLLL FPSTYLCETG FSTLSVIKTK HRNSLNIHYP LRVALSSIQP RLDKLTSKKQ1321AHLSHTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1325 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.