Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ZBED8 / Buster3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G110459-100
Catalog Number: LS-G110459
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C5orf54(ZBED8) (NM_022090), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- C5orf54 | Buster3
- Origin Species
- Human
- NCBI GenBank
- AF205598
- SwissProt
- Q8IZ13
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 68.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSKKRKWDDD YVRYWFTCTT EVDGTQRPQC VLCNSVFSNA DLRPSKLSDH FNRQHGGVAG61HDLNSLKHMP APSDQSETLK AFGVASHEDT LLQASYQFAY LCAKEKNPHT VAEKLVKPCA121LEIAQIVLGP DAQKKLQQVP LSDDVIHSRI DEMSQDILQQ VLEDIKASPL KVGIQLAETT181DMDDCSQLMA FVRYIKEREI VEEFLFCEPL QLSMKGIDVF NLFRDFFLKH KIALDVCGSV241CTDGASSMLG ENSEFVAYVK KEIPHIVVTH CLLNPHALVI KTLPTKLRDA LFTVVRVINF301IKGRAPNHRL FQAFFEEIGI EYSVLLFHTE MRWLSRGQIL THIFEMYEEI NQFLHHKSSN361LVDGFENKEF KIHLAYLADL FKHLNELSAS MQRTGMNTVS AREKLSAFVR KFPFWQKRIE421KRNFTNFPFL EEIIVSDNEG IFIAAEITLH LQQLSNFFHG YFSIGDLNEA SKWILDPFLF481NIDFVDDSYL MKNDLAELRA SGQILMEFET MKLEDFWCAQ FTAFPNLAKT ALEILMPFAT541TYLCELGFSS LLHFKTKSRS CFNLSDDIRV AISKKVPRFS DIIEQKLQLQ QKSLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
594 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.