LSBio
ZBED4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZBED4 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ZBED4 antibodies →
$725.00each
Catalog Number: LS-G113659-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZBED4 (NM_014838), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ZBED4 | KIAA0637
Origin Species
Human
NCBI GenBank
AB014537
SwissProt
O75132

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
130.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MENNLKTCPK EDGDFVSDKI KFKIEEEDDD GIPPDSLERM DFKSEQEDMK QTDSGGERAG
61LGGTGCSCKP PGKYLSAESE DDYGALFSQY SSTLYDVAME AVTQSLLSSR NMSSRKKSPA
121WKHFFISPRD STKAICMYCV KEFSRGKNEK DLSTSCLMRH VRRAHPTVLI QENGSVSAVS
181SFPSPSLLLP PQPADAGDLS TILSPIKLVQ KVASKIPSPD RITEESVSVV SSEEISSDMS
241VSEKCGREEA LVGSSPHLPA LHYDEPAENL AEKSLPLPKS TSGSRRRSAV WKHFYLSPLD
301NSKAVCIHCM NEFSRGKNGK DLGTSCLIRH MWRAHRAIVL QENGGTGIPP LYSTPPTLLP
361SLLPPEGELS SVSSSPVKPV RESPSASSSP DRLTEDLQSH LNPGDGLMED VAAFSSSDDV
421GEASASSPEK QQADGLSPRL FESGAIFQQN KKVMKRLKSE VWHHFSLAPM DSLKAECRYC
481GCAISRGKKG DVGTSCLMRH LYRRHPEVVG SQKGFLGASL ANSPYATLAS AESSSSKLTD
541LPTVVTKNNQ VMFPVNSKKT SKLWNHFSIC SADSTKVVCL HCGRTISRGK KPTNLGTSCL
601LRHLQRFHSN VLKTEVSETA RPSSPDTRVP RGTELSGASS FDDTNEKFYD SHPVAKKITS
661LIAEMIALDL QPYSFVDNVG FNRLLEYLKP QYSLPAPSYF SRTAIPGMYD NVKQIIMSHL
721KEAESGVIHF TSGIWMSNQT REYLTLTAHW VSFESPARPR CDDHHCSALL DVSQVDCDYS
781GNSIQKQLEC WWEAWVTSTG LQVGITVTDN ASIGKTLNEG EHSSVQCFSH TVNLIVSEAI
841KSQRMVQNLL SLARKICERV HRSPKAKEKL AELQREYALP QHHLIQDVPS KWSTSFHMLE
901RLIEQKRAIN EMSVECNFRE LISCDQWEVM QSVCRALKPF EAASREMSTQ MSTLSQVIPM
961VHILNRKVEM LFEETMGIDT MLRSLKEAMV SRLSATLHDP RYVFATLLDP RYKASLFTEE
1021EAEQYKQDLI RELELMNSTS EDVAASHRCD AGSPSKDSAA EENLWSLVAK VKKKDPREKL
1081PEAMVLAYLE EEVLEHSCDP LTYWNLKKAS WPGLSALAVR FLGCPPSIVP SEKLFNTPTE
1141NGSLGQSRLM MEHFEKLIFL KVNLPLIYFQ Y
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1171 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.