Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ZBED1 / TRAMP Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G116459-100
Catalog Number: LS-G116459
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DREF(ZBED1) (NM_004729), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Ac-like transposable element | ALTE | DREF homolog | HDREF | ZBED1 | DREF | KIAA0785 | TRAMP
- Origin Species
- Human
- NCBI GenBank
- NM_004729
- SwissProt
- O96006
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 78 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MENKSLESSQ TDLKLVAHPR AKSKVWKYFG FDTNAEGCIL QWKKIYCRIC MAQIAYSGNT61SNLSYHLEKN HPEEFCEFVK SNTEQMREAF ATAFSKLKPE SSQQPGQDAL AVKAGHGYDS121KKQQELTAAV LGLICEGLYP ASIVDEPTFK VLLKTADPRY ELPSRKYIST KAIPEKYGAV181REVILKELAE ATWCGISTDM WRSENQNRAY VTLAAHFLGL GAPNCLSMGS RCLKTFEVPE241ENTAETITRV LYEVFIEWGI SAKVFGATTN YGKDIVKACS LLDVAVHMPC LGHTLNAGIQ301QAFQLPKLGA LLSRCRKLVE YFQQSAVAMY MLYEKQKQQN VAHCMLVSNR VSWWGSTLAM361LQRLKEQQFV IAGVLVEDSN NHHLMLEASE WATIEGLVEL LQPFKQVAEM LSASRYPTIS421MVKPLLHMLL NTTLNIKETD SKELSMAKEV IAKELSKTYQ ETPEIDMFLN VATFLDPRYK481RLPFLSAFER QQVENRVVEE AKGLLDKVKD GGYRPAEDKI FPVPEEPPVK KLMRTSTPPP541ASVINNMLAE IFCQTGGVED QEEWHAQVVE ELSNFKSQKV LGLNEDPLKW WSDRLALFPL601LPKVLQKYWC VTATRVAPER LFGSAANVVS AKRNRLAPAH VDEQVFLYEN ARSGAEAEPE661DQDEGEWGLD QEQVFSLGDG VSGGFFGIRD SSFLTRTRPL EQKLISEEDL AANDILDYKD721DDDKV
725 aa
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.