LSBio
ZBBX Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZBBX Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ZBBX antibodies →
$725.00each
Catalog Number: LS-G109837-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZBBX (NM_024687), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ZBBX
Origin Species
Human
NCBI GenBank
NM_024687
SwissProt
A8MT70

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
91.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MNRKDFVVLP WGKPGNSVKL KYRNAQELRM EKVQLEFENQ EMEKKLQEFR STRNKEKEDR
61ESSEYYWKSG KVGKLVNQSY MMSQNKGNVV KFSAGKVKLK LLKEQIQEPV KPTVNYKMAN
121SSECEKPKIN GKVCGQCENK AALLVCLECG EDYCSGCFAK VHQKGALKLH RTTLLQAKSQ
181ILFNVLDVAH QFIKDVNPDE PKEENNSTKE TSKIQHKPKS VLLQRSSSEV EITTMKRAQR
241TKPRKSLLCE GSFDEEASAQ SFQEVLSQWR TGNHDDNKKQ NLHAAVKDSL EECEVQTNLK
301IWREPLNIEL KEDILSYMEK LWLKKHRRTP QEQLFKMLPD TFPHPHETTG DAQCSQNEND
361EDSDGEETKV QHTALLLPVE TLNIERPEPS LKIVELDDTY EEEFEEAENI VPYKVKLADA
421DSQRSCAFHD CQKNSFPYEN GIHQHHVFDK GKRDFLNLCL RNSSTYYKDN SKAETSNTDF
481DNIVDPDVYS SDIEKIEEST SFERNLKEKN IGLESNQKSD DSCVSLESKD TLLGRDLEKA
541PIEEKLSQDI KESLELSNLY KRPSFEESKT TKSSLLLQEI ACRSKPITKQ YQGLERFFIF
601DTNERLNLLP SHRLECNNSS TRITLAGQKS QRPSTANFPL SNSVKESSSC LSSSHPRSRS
661AAAQSSSRAA SEISEIEYID ITDQNELSLD DTTDQHTLDN LEKELQVLRS LADTSEKLYS
721LTSEEFPDFS SQSLNISQIS TDFLKTSHVR GPCGVEELSC SGRDTKIQSL LSLSESSTDE
781EEEDFLNKQH VITLPWSKST
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

800 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.