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YY1AP1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

YY1AP1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all YY1AP1 antibodies →
$504.00each
Catalog Number: LS-G107133-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human YY1AP1 (NM_139119), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HCCA2 | YAP | YY1 associated protein 1 | YY1AP1 | HCCA1 | YY1-associated protein 1 | YY1AP
Origin Species
Human
NCBI GenBank
NM_018253
SwissProt
Q9H869

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
82.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEDLFETFQD EMGFSNMEDD GPEEEERVAE PQANFNTPQA LRFEELLANL LNEQHQIAKE
61LFEQLKMKKP SAKQQKEVEK VKPQCKEVHQ TLILDPAQRK RLQQQMQQHV QLLTQIHLLA
121TCNPNLNPEA SSTRICLKEL GTFAQSSIAL HHQYNPKFQT LFQPCNLMGA MQLIEDFSTH
181VSIDCSPHKT VKKTANEFPC LPKQVAWILA TSKVFMYPEL LPVCSLKAKN PQDKILFTKA
241EDNLLALGLK HFEGTEFLNP LISKYLLTCK TARQLTVRIK NLNMNRAPDN IIKFYKKTKQ
301LPVLGKCCEE IQPHQWKPPI EREEHRLPFW LKASLPSIQE ELRHMADGAR EVGNMTGTTE
361INSDQGLEKD NSELGSETRY PLLLPKGVVL KLKPVADRFP KKAWRQKRSS VLKPLLIQPS
421PSLQPSFNPG KTPAQSTHSE APPSKMVLRI PHPIQPATVL QTVPGVPPLG VSGGESFESP
481AALPAMPPEA RTSFPLSESQ TLLSSAPVPK VMMPSPASSM FRKPYVRRRP SKRRGARAFR
541CIKPAPVIHP ASVIFTVPAT TVKIVSLGGG CNMIQPVNAA VAQSPQTIPI ATLLVNPTSF
601PCPLNQPLVA SSVSPLIVSG NSVNLPIPST PEDKAHMNVD IACAVADGEN AFQGLEPKLE
661PQELSPLSAT VFPKVEHSPG PPPVDKQCQE GLSENSAYRW TVVKTEEGRQ ALEPLPQGIQ
721ESLNNSSPGD LEEVVKMEPE DATEEISGFL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

750 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.