Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Yo / CDR2 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G118391-100
Catalog Number: LS-G118391
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CDR2 (NM_001802), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CDR2 | CDR62 | Yo paraneoplastic antigen | PCD17 | Yo
- Origin Species
- Human
- NCBI GenBank
- M63256
- SwissProt
- Q01850
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 51.7 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MLAENLVEEF EMKEDEPWYD HQDLQQDLQL AAELGKTLLD RNTELEDSVQ QMYTTNQEQL61QEIEYLTKQV ELLRQMNEQH AKVYEQLDVT ARELEETNQK LVADSKASQQ KILSLTETIE121CLQTNIDHLQ SQVEELKSSG QGRRSPGKCD QEKPAPSFAC LKELYDLRQH FVYDHVFAEK181ITSLQGQPSP DEEENEHLKK TVTMLQAQLS LERQKRVTME EEYGLVLKEN SELEQQLGAT241GAYRARALEL EAEVAEMRQM LQSEHPFVNG VEKLVPDSLY VPFKEPSQSL LEEMFLTVPE301SHRKPLKRSS SETILSSLAG SDIVKGHEET CIRRAKAVKQ RGISLLHEVD TQYSALKVKY361EELLKKCQEE QDSLSHKAVQ TSRAAAKDLT GVNAQSEPVA SGWELASVNP EPVSSPTTPP421EYKALFKEIF SCIKKTKQEI DEQRTKYRSL SSHSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
454 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.