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YME1L1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

YME1L1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all YME1L1 antibodies →
$504.00each
Catalog Number: LS-G114056-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ATP dependent metalloprotease YME1L1(YME1L1) (NM_014263), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
FTSH | FTSH1 | Meg-4 | YME1 (S.cerevisiae)-like 1 | YME1-like 1 (S. cerevisiae) | YME1-like protein 1 | MEG4 | PAMP | YME1L | YME1L1
Origin Species
Human
NCBI GenBank
NM_014263
SwissProt
Q96TA2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
79.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MFSLSSTVQP QVTVPLSHLI NAFHTPKNTS VSLSGVSVSQ NQHRDVVPEH EAPSSEPSLN
61LRDLGLSELK IGQIDQLVEN LLPGFCKGKN ISSHWHTSHV SAQSFFENKY GNLDIFSTLR
121SSCLYRHHSR ALQSICSDLQ YWPVFIQSRG FKTLKSRTRR LQSTSERLAE TQNIAPSFVK
181GFLLRDRGSD VESLDKLMKT KNIPEAHQDA FKTGFAEGFL KAQALTQKTN DSLRRTRLIL
241FVLLLFGIYG LLKNPFLSVR FRTTTGLDSA VDPVQMKNVT FEHVKGVEEA KQELQEVVEF
301LKNPQKFTIL GGKLPKGILL VGPPGTGKTL LARAVAGEAD VPFYYASGSE FDEMFVGVGA
361SRIRNLFREA KANAPCVIFI DELDSVGGKR IESPMHPYSR QTINQLLAEM DGFKPNEGVI
421IIGATNFPEA LDNALIRPGR FDMQVTVPRP DVKGRTEILK WYLNKIKFDQ SVDPEIIARG
481TVGFSGAELE NLVNQAALKA AVDGKEMVTM KELEFSKDKI LMGPERRSVE IDNKNKTITA
541YHESGHAIIA YYTKDAMPIN KATIMPRGPT LGHVSLLPEN DRWNETRAQL LAQMDVSMGG
601RVAEELIFGT DHITTGASSD FDNATKIAKR MVTKFGMSEK LGVMTYSDTG KLSPETQSAI
661EQEIRILLRD SYERAKHILK THAKEHKNLA EALLTYETLD AKEIQIVLEG KKLEVR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

716 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.