Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
YKL39 / CHI3L2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G121141-100
Catalog Number: LS-G121141
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CHI3L2 (NM_001025197), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Chondrocyte protein 39 | Chitinase 3-like 2 | YKL-39 | CHI3L2 | Chitinase-3-like protein 2 | YKL39
- Origin Species
- Human
- NCBI GenBank
- NM_004000
- SwissProt
- Q15782
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 42.3 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MGATTMDQKS LWAGSAYKLV CYFTNWSQDR QEPGKFTPEN IDPFLCSHLI YSFASIENNK61VIIKDKSEVM LYQTINSLKT KNPKLKILLS IGGYLFGSKG FHPMVDSSTS RLEFINSIIL121FLRNHNFDGL DVSWIYPDQK ENTHFTVLIH ELAEAFQKDF TKSTKERLLL TVGVSAGRQM181IDNSYQVEKL AKDLDFINLL SFDFHGSWEK PLITGHNSPL SKGWQDRGPS SYYNVEYAVG241YWIHKGMPSE KVVMGIPTYG HSFTLASAET TVGAPASGPG AAGPITESSG FLAYYEICQF301LKGAKITRLQ DQQVPYAVKG NQWVGYDDVK SMETKVQFLK NLNLGGAMIW SIDMDDFTGK361SCNQGPYPLV QAVKRSLGSLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
380 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.