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XRN1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

XRN1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all XRN1 antibodies →
$725.00each
Catalog Number: LS-G111519-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human XRN1 (NM_019001), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
5'-3' exoribonuclease 1 | 5-3 exoribonuclease 1 | SEP1 | XRN1
Origin Species
Human
NCBI GenBank
AL133623
SwissProt
Q8IZH2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
193.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGVPKFYRWI SERYPCLSEV VKEHQIPEFD NLYLDMNGII HQCSHPNDDD VHFRISDDKI
61FTDIFHYLEV LFRIIKPRKV FFMAVDGVAP RAKMNQQRGR RFRSAKEAED KIKKAIEKGE
121TLPTEARFDS NCITPGTEFM ARLHEHLKYF VNMKISTDKS WQGVTIYFSG HETPGEGEHK
181IMEFIRSEKA KPDHDPNTRH CLYGLDADLI MLGLTSHEAH FSLLREEVRF GGKKTQRVCA
241PEETTFHLLH LSLMREYIDY EFSVLKEKIT FKYDIERIID DWILMGFLVG NDFIPHLPHL
301HINHDALPLL YGTYVTILPE LGGYINESGH LNLPRFEKYL VKLSDFDREH FSEVFVDLKW
361FESKVGNKYL NEAAGVAAEE ARNYKEKKKL KGQENSLCWT ALDKNEGEMI TSKDNLEDET
421EDDDLFETEF RQYKRTYYMT KMGVDVVSDD FLADQAACYV QAIQWILHYY YHGVQSWSWY
481YPYHYAPFLS DIHNISTLKI HFELGKPFKP FEQLLAVLPA ASKNLLPACY QHLMTNEDSP
541IIEYYPPDFK TDLNGKQQEW EAVVLIPFID EKRLLEAMET CNHSLKKEER KRNQHSECLM
601CWYDRDTEFI YPSPWPEKFP AIERCCTRYK IISLDAWRVD INKNKITRID QKALYFCGFP
661TLKHIRHKFF LKKSGVQVFQ QSSRGENMML EILVDAESDE LTVENVASSV LGKSVFVNWP
721HLEEARVVAV SDGETKFYLE EPPGTQKLYS GRTAPPSKVV HLGDKEQSNW AKEVQGISEH
781YLRRKGIIIN ETSAVVYAQL LTGRKYQINQ NGEVRLEKQW SKQVVPFVYQ TIVKDIRAFD
841SRFSNIKTLD DLFPLRSMVF MLGTPYYGCT GEVQDSGDVI TEGRIRVIFS IPCEPNLDAL
901IQNQHKYSIK YNPGYVLASR LGVSGYLVSR FTGSIFIGRG SRRNPHGDHK ANVGLNLKFN
961KKNEEVPGYT KKVGSEWMYS SAAEQLLAEY LERAPELFSY IAKNSQEDVF YEDDIWPGEN
1021ENGAEKVQEI ITWLKGHPVS TLSRSSCDLQ ILDAAIVEKI EEEVEKCKQR KNNKKVRVTV
1081KPHLLYRPLE QQHGVIPDRD AEFCLFDRVV NVRENFSVPV GLRGTIIGIK GANREADVLF
1141EVLFDEEFPG GLTIRCSPGR GYRLPTSALV NLSHGSRSET GNQKLTAIVK PQPAVHQHSS
1201SSSVSSGHLG ALNHSPQSLF VPTQVPTKDD DEFCNIWQSL QGSGKMQYFQ PTIQEKGAVL
1261PQEISQVNQH HKSGFNDNSV KYQQRKHDPH RKFKEECKSP KAECWSQKMS NKQPNSGIEN
1321FLASLNISKE NEVQSSHHGE PPSEEHLSPQ SFAMGTRMLK EILKIDGSNT VDHKNEIKQI
1381ANEIPVSSNR RDEYGLPSQP KQNKKLASYM NKPHSANEYH NVQSMDNMCW PAPSQIPPVS
1441TPVTELSRIC SLVGMPQPDF SFLRMPQTMT VCQVKLSNGL LVHGPQCHSE NEAKEKAALF
1501ALQQLGSLGM NFPLPSQVFA NYPSAVPPGT IPPAFPPPTG WDHYGSNYAL GAANIMPSSS
1561HLFGSMPWGP SVPVPGKPFH HTLYSGTMPM AGGIPGGVHN QFIPLQVTKK RVANKKNFEN
1621KEAQSSQATP VQTSQPDSSN IVKVSPRESS SASLKSSPIA QPASSFQVET ASQGHSISHH
1681KSTPISSSRR KSRKLAVNFG VSKPSE
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1706 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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