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XPO7 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

XPO7 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all XPO7 antibodies →
$504.00each
Catalog Number: LS-G101286-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RanBP16(XPO7) (NM_015024), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_015024

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
123.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MADHVQSLAQ LENLCKQLYE TTDTTTRLQA EKALVEFTNS PDCLSKCQLL LERGSSSYSQ
61LLAATCLTKL VSRTNNPLPL EQRIDIRNYV LNYLATRPKL ATFVTQALIQ LYARITKLGW
121FDCQKDDYVF RNAITDVTRF LQDSVEYCII GVTILSQLTN EINQADTTHP LTKHRKIASS
181FRDSSLFDIF TLSCNLLKQA SGKNLNLNDE SQHGLLMQLL KLTHNCLNFD FIGTSTDESS
241DDLCTVQIPT SWRSAFLDSS TLQLFFDLYH SIPPSFSPLV LSCLVQIASV RRSLFNNAER
301AKFLSHLVDG VKRILENPQS LSDPNNYHEF CRLLARLKSN YQLGELVKVE NYPEVIRLIA
361NFTVTSLQHW EFAPNSVHYL LSLWQRLAAS VPYVKATDPH MLETYTPEVT KAYITSRLES
421VHIILRDGLE DPLEDTGLVQ QQLDQLSTIG RCEYEKTCAL LVQLFDQSAQ SYQELLQSAS
481ASPMDIAVQE GRLTWLVYII GAVIGGRVSF ASTDEQDAMD GELVCRVLQL MNLTDSRLAQ
541AGNEKLELAM LSFFEQFRKI YIGDQVQKSS KLYRRLSEVL GLNDETMVLS VFIGKIITNL
601KYWGRCEPIT SKTLQLLNDL SIGYSSVRKL VKLSAVQFML NNHTSEHFSF LGINNQSNLT
661DMRCRTTFYT ALGRLLMVDL GEDEDQYEQF MLPLTAAFEA VAQMFSTNSF NEQEAKRTLV
721GLVRDLRGIA FAFNAKTSFM MLFEWIYPSY MPILQRAIEL WYHDPACTTP VLKLMAELVH
781NRSQRLQFDV SSPNGILLFR ETSKMITMYG NRILTLGEVP KDQVYALKLK GISIYFSMLK
841AALSGSYVNF GVFRLYGDDA LDNALQTFIK LLLSIPHSDL LDYPKLSQSY YSLLEVLTQD
901HMNFIASLEP HVIMYILSSI SEGLTALDTM VCTGCCSCLD HIVTYLFKQL SRSTKKRTTP
961LNQESDRFLH IMQQHPEMIQ QMLSTVLNII IFEDCRNQWS MSRPLLGLIL LNEKYFSDLR
1021NSIVNSQPPE KQQAMHLCFE NLMEGIERNL LTKNRDRFTQ NLSAFRREVN DSMKNSTYGV
1081NSNDMMS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1087 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.