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XPO6 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

XPO6 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all XPO6 antibodies →
$725.00each
Catalog Number: LS-G113431-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human XPO6 (NM_015171), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
EXP6 | Exportin 6 | Exportin-6 | KIAA0370 | RAN binding protein 20 | XPO6 | Ran-binding protein 20 | RANBP20
Origin Species
Human
NCBI GenBank
AY026388
SwissProt
Q96QU8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
128.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MASEEASLRA LESLMTEFFH DCTTNERKRE IEELLNNFAQ QIGAWRFCLY FLSSTRNDYV
61MMYSLTVFEN LINKMWLGVP SQDKMEIRSC LPKLLLAHHK TLPYFIRNKL CKVIVDIGRQ
121DWPMFYHDFF TNILQLIQSP VTTPLGLIML KTTSEELACP REDLSVARKE ELRKLLLDQV
181QTVLGLLTGI LETVWDKHSV TAATPPPSPT SGESGDLLSN LLQSPSSAKL LNQPIPILDV
241ESEYICSLAL ECLAHLFSWI PLSASITPSL LTTIFHFARF GCDIRARKMA SVNGSSQNCV
301SGQERGRLGV LAMSCINELM SKNCVPMEFE EYLLRMFQQT FYLLQKITKD NNAHTVKSRL
361EELDESYIEK FTDFLRLFVS VHLRRIESYS QFPVVEFLTL LFKYTFHQPT HEGYFSCLDI
421WTLFLDYLTS KIKSRLGDKE AVLNRYEDAL VLLLTEVLNR IQFRYNQAQL EELDDETLDD
481DQQTEWQRYL RQSLEVVAKV MELLPTHAFS TLFPVLQDNL EVYLGLQQFI VTSGSGHRLN
541ITAENDCRRL HCSLRDLSSL LQAVGRLAEY FIGDVFAARF NDALTVVERL VKVTLYGSQI
601KLYNIETAVP SVLKPDLIDV HAQSLAALQA YSHWLAQYCS EVHRQNTQQF VTLISTTMDA
661ITPLISTKVQ DKLLLSACHL LVSLATTVRP VFLISIPAVQ KVFNRITDAS ALRLVDKAQV
721LVCRALSNIL LLPWPNLPEN EQQWPVRSIN HASLISALSR DYRNLKPSAV APQRKMPLDD
781TKLIIHQTLS VLEDIVENIS GESTKSRQIC YQSLQESVQV SLALFPAFIH QSDVTDEMLS
841FFLTLFRGLR VQMGVPFTEQ IIQTFLNMFT REQLAESILH EGSTGCRVVE KFLKILQVVV
901QEPGQVFKPF LPSIIALCME QVYPIIAERP SPDVKAELFE LLFRTLHHNW RYFFKSTVLA
961SVQRGIAEEQ MENEPQFSAI MQAFGQSFLQ PDIHLFKQNL FYLETLNTKQ KLYHKKIFRT
1021AMLFQFVNVL LQVLVHKSHD LLQEEIGIAI YNMASVDFDG FFAAFLPEFL TSCDGVDANQ
1081KSVLGRNFKM DRDLPSFTQN VHRLVNDLRY YRLCNDSLPP GTVKL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1125 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.