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XAGE1E Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

XAGE1E Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G119027-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human XAGE1(XAGE1B) (NM_001097596), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CT12.1D | CTP9 | GAGED2 | G antigen family D member 2 | XAGE1 | X antigen family | member 1D | XAGE1D | Cancer/testis antigen 12.1 | CT12.1 | Protein XAGE-1
Origin Species
Human
NCBI GenBank
NM_020411
SwissProt
Q9HD64

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MESPKKKNQQ LKVGILHLGS RQKKIRIQLR SQVLGREMRD MEGDLQELHQ SNTGDKSGFG
61FRRQGEDNT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

69 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.