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WWC3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

WWC3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G113056-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human WWC3 (NM_015691), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BM042 | KIAA1280 | Protein WWC3 | WWC family member 3 | WWC3
Origin Species
Human
NCBI GenBank
NM_015691
SwissProt
Q9ULE0

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
122.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDHINKLTQI EDPREQWRRE QERMLKEYLI VAQEALNAKK EIYQIKQQRF ELAQEEYQQL
61HKMCEDDSRS YASSFSGYST NTKYDPHQIK AEIASRRDRL SRLKRELTQM KQELQYKEKG
121VETLQEIDRK MSSTHTSYKL DEAQAIMSEL RTIKKAICTG EKERRDLMHS LAKLTDSFKN
181SCSVTDSLVD FPHHVGVPGD AGVPQQFCDA GSQTDIIGEF VFDDKTRLVD RVRLNWQYEE
241ARKRVANIQQ QLARLDNESW PSTAEADRDR LQLIKEKEAL LQELQLIIAQ RRSAGDVARL
301EEERERLEEE LRRARATSAQ GATERILLQE KRNCLLMQLE EATRLTSYLQ SQLKSLCAST
361LTVSSGSSRG SLASSRGSLA SSRGSLSSVS FTDIYGLPQY EKPDAEGSQL LRFDLIPFDS
421LGRDAPFSEP PGPSGFHKQR RSLDTPQSLA SLSSRSSLSS LSPPSSPLDT PFLPASRDSP
481LAQLADSCEG PGLGALDRLR AHASAMGDED LPGMAALQPH GVPGDGEGPH ERGPPPASAP
541VGGTVTLRED SAKRLERRAR RISACLSDYS LASDSGVFEP LTKRNEDAEE PAYGDTASNG
601DPQIHVGLLR DSGSECLLVH VLQLKNPAGL AVKEDCKVHI RVYLPPLDSG TPNTYCSKAL
661EFQVPLVFNE VFRIPVHSSA LTLKSLQLYV CSVTPQLQEE LLGIAQINLA DYDSLSEMQL
721RWHSVQVFTS SEPSRTREAG CAGESSARDP AHTISISGKT DAVTVLLART TAQLQAVERE
781LAEERAKLEY TEEEVLEMER KEEQAEAISE RSWQADSVDS GCSNCTQTSP PYPEPCCMGI
841DSILGHPFAA QAGPYSPEKF QPSPLKVDKE TNTEDLFLEE AASLVKERPS RRARGSPFVR
901SGTIVRSQTF SPGARSQYVC RLYRSDSDSS TLPRKSPFVR NTLERRTLRY KQSCRSSLAE
961LMARTSLDLE LDLQASRTRQ RQLNEELCAL RELRQRLEDA QLRGQTDLPP WVLRDERLRG
1021LLREAERQTR QTKLDYRHEQ AAEKMLKKAS KEIYQLRGQS HKEPIQVQTF REKIAFFTRP
1081RINIPPLPAD DV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1092 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.