Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
WRAP73 / WDR8 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G112202-100
Catalog Number: LS-G112202
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human WDR8(WRAP73) (NM_017818), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- WRAP73 | WD repeat domain 8 | WD repeat-containing protein 8 | WDR8
- Origin Species
- Human
- NCBI GenBank
- NM_017818
- SwissProt
- Q9P2S5
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 51.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MNFSEVFKLS SLLCKFSPDG KYLASCVQYR LVVRDVNTLQ ILQLYTCLDQ IQHIEWSADS61LFILCAMYKR GLVQVWSLEQ PEWHCKIDEG SAGLVASCWS PDGRHILNTT EFHLRITVWS121LCTKSVSYIK YPKACLQGIT FTRDGRYMAL AERRDCKDYV SIFVCSDWQL LRHFDTDTQD181LTGIEWAPNG CVLAVWDTCL EYKILLYSLD GRLLSTYSAY EWSLGIKSVA WSPSSQFLAV241GSYDGKVRIL NHVTWKMITE FGHPAAINDP KIVVYKEAEK SPQLGLGCLS FPPPRAGAGP301LPSSESKYEI ASVPVSLQTL KPVTDRANPK IGIGMLAFSP DSYFLATRND NIPNAVWVWD361IQKLRLFAVL EQLSPVRAFQ WDPQQPRLAI CTGGSRLYLW SPAGCMSVQV PGEGDFAVLS421LCWHLSGDSM ALLSKDHFCL CFLETEAVVG TACRQLGGHTTRTRPLEQKLISEEDLAANDILDYKDDDDKV
460 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.