Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
WNT2 / IRP Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G100037-100
Catalog Number: LS-G100037
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human WNT2 (NM_003391), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- IRP | Int-1-like protein 1 | Int-1-related protein | Protein Wnt-2 | WNT2 | Secreted growth factor | INT1L1
- Origin Species
- Human
- NCBI GenBank
- NM_003391
- SwissProt
- P09544
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 40.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MNAPLGGIWL WLPLLLTWLT PEVNSSWWYM RATGGSSRVM CDNVPGLVSS QRQLCHRHPD61VMRAISQGVA EWTAECQHQF RQHRWNCNTL DRDHSLFGRV LLRSSRESAF VYAISSAGVV121FAITRACSQG EVKSCSCDPK KMGSAKDSKG IFDWGGCSDN IDYGIKFARA FVDAKERKGK181DARALMNLHN NRAGRKAVKR FLKQECKCHG VSGSCTLRTC WLAMADFRKT GDYLWRKYNG241AIQVVMNQDG TGFTVANERF KKPTKNDLVY FENSPDYCIR DREAGSLGTA GRVCNLTSRG301MDSCEVMCCG RGYDTSHVTR MTKCGCKFHW CCAVRCQDCL EALDVHTCKA PKNADWTTATTRTRPLEQKLISEEDLAANDILDYKDDDDKV
360 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.