Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
WLS / GPR177 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G130962-100
Catalog Number: LS-G130962
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GPR177(WLS) (NM_001193334), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- C1orf139 | G protein-coupled receptor 177 | Protein wntless homolog | MRP | WLS | Wntless homolog (Drosophila) | EVI | GPR177
- Origin Species
- Human
- NCBI GenBank
- NM_024911
- SwissProt
- Q5T9L3
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 52 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAGAIIENMS TKKLCIVGGI LLVFQIIAFL VGGLIGENAE VSMDVSLAYR DDAFAEWTEM61AHERVPRKLK CTFTSPKTPE HEGRYYECDV LPFMEIGSVA HKFYLLNIRL PVNEKKKINV121GIGEIKDIRL VGIHQNGGFT KVWFAMKTFL TPSIFIIMVW YWRRITMMSR PPVLLEKVIF181ALGISMTFIN IPVEWFSIGF DWTWMLLFGD IRQGIFYAML LSFWIIFCGE HMMDQHERNH241IAGYWKQVGP IAVGSFCLFI FDMCERGVQL TNPFYSIWTT DIGTELAMAF IIVAGICLCL301YFLFLCFMVF QVFRNISGKQ SSLPAMSKVR RLHYEGLIFR FKFLMLITLA CAAMTVIFFI361VSQVTEGHWK WGGVTVQVNS AFFTGIYGMW NLYVFALMFL YAPSHKNYGE DQSNGDLGVH421SGEELQLTTT ITHVDGPTEI YKLTRKEAQESGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
450 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.