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WIPI2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

WIPI2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G120923-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human WIPI2 (NM_001033520), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ATG18B | Atg21 | DKFZP434J154 | WIPI-2 | WIPI2 | WIPI49-like protein 2 | CGI-50
Origin Species
Human
NCBI GenBank
NM_016003

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
41.9 kda
Protein Type
Lysate
Amino Acid Sequence
1MFTHVLPFVI SADTEDVCIV ERLFSSSLVA IVSLKAPRKL KVCHFKKGTE ICNYSYSNTI
61LAVKLNRQRL IVCLEESLYI HNIRDMKVLH TIRETPPNPA GLCALSINND NCYLAYPGSA
121TIGEVQVFDT INLRAANMIP AHDSPLAALA FDASGTKLAT ASEKGTVIRV FSIPEGQKLF
181EFRRGVKRCV SICSLAFSMD GMFLSASSNT ETVHIFKLET VKEKPPEEPT TWTGYFGKVL
241MASTSYLPSQ VTEMFNQGRA FATVRLPFCG HKNICSLATI QKIPRLLVGA ADGYLYMYNL
301DPQEGGECAL MKQHRLDGSL ETTNEILDSA SHDCPLVTQT YGAAAGKAYT DDLGAVGGAC
361LEDEASALRL DEDSEHPPMI LRTD
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

384 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.