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WIPF3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

WIPF3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G119686-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CR16(WIPF3) (NM_001080529), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
WIPF3 | CR16
Origin Species
Human
SwissProt
A6NGB9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
49.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPVPPPPPPP LPPPPPPLGA PPPPPPSAPP VSTDTSSLRR ADPKGRSALL ADIQQGTRLR
61KVTQINDRSA PQIESSKGTN KEGGGSANTR GASTPPTLGD LFAGGFPVLR PAGQRDVAGG
121KTGQGPGSRA PSPRLPNKTI SGPLIPPASP RLGNTSEAHG AARTAPPRPN VPAPPPPTPP
181PPPPPLPPPL PSSSPIKTPL VSPPGPLTKG NLPVVAPPVP CAPPPPPPPP PPTPPPLPPA
241SVLSDKAVKP QLAPLHLPPI PPPLPLLPPC GYPGLKAEPA SPAQDAQEPP APPPPLPPYA
301SCSPRASLPA PPLPGVNSSS ETPPPLPPKS PSFQAPPQKA GAQALPAPPA PPGSQPFLQK
361KRHGRPGAGG GKLNPPPAPP ARSPTTELSS KSQQATAWTP TQQPGGQLRN GSLHIIDDFE
421SKFTFHSVED FPPPDEYKPC QKIYPSKIPR SRTPGPWLQA EAVGQSSDDI KGRNSQVTQA
481SCL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

483 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.