Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
WIPF2 / WIRE Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G107560-100
Catalog Number: LS-G107560
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human WIPF2 (NM_133264), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- WASP-binding protein | WIP-related protein | WICH | WIPF2 | WIRE
- Origin Species
- Human
- SwissProt
- Q8TF74
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 46.1 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MPIPPPPPPP PGPPPPPTFH QANTEQPKLS RDEQRGRGAL LQDICKGTKL KKVTNINDRS61APILEKPKGS SGGYGSGGAA LQPKGGLFQG GVLKLRPVGA KDGSENLAGK PALQIPSSRA121AAPRPPVSAA SGRPQDDTDS SRASLPELPR MQRPSLPDLS RPNTTSSTGM KHSSSAPPPP181PPGRRANAPP TPLPMHSSKA PAYNREKPLP PTPGQRLHPG REGPPAPPPV KPPPSPVNIR241TGPSGQSLAP PPPPYRQPPG VPNGPSSPTN ESAPELPQRH NSLHRKTPGP VRGLAPPPPT301SASPSLLSNR PPPPARDPPS RGAAPPPPPP VIRNGARDAP PPPPPYRMHG SEPPSRGKPP361PPPSRTPAGP PPPPPPPLRN GHRDSITTVR SFLDDFESKY SFHPVEDFPA PEEYKHFQRI421YPSKTNRAAR GAPPLPPILRTRTRPLEQKLISEEDLAANDILDYKDDDDKV
440 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.