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WHAMM Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

WHAMM Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all WHAMM antibodies →
$725.00each
Catalog Number: LS-G120221-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human WHAMM (NM_001080435), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA1971 | WHAMM | WHDC1
Origin Species
Human
SwissProt
Q8TF30

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
90.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEDEQPDSLE GWVPVREGLF AEPERHRLRF LVAWNGAEGK FAVTCHDRTA QQRRLREGAR
61LGPEPEPKPE AAVSPSSWAG LLSAAGLRGA HRQLAALWPP LERCFPRLPP ELDVGGGGAW
121GLGLGLWALL WPTRAGPGEA ALQELCGQLE RYLGAAADGC GGATVRDALF PAEGGAADCE
181SPREFRERAL RARWVEADAR LRQVIQGHGK ANTMVALMNV YQEEDEAYQE LVTVATMFFQ
241YLLQPFRAMR EVATLCKLDI LKSLDEDDLG PRRVVALEKE AEEWTRRAEE AVVSIQDITV
301NYFKETVKAL AGMQKEMEQD AKRFGQAAWA TAIPRLEKLQ LMLARETLQL MRAKELCLNH
361KRAEIQGKME DLPEQEKNTN VVDELEIQFY EIQLELYEVK FEILKNEEIL LTTQLDSLKR
421LIKEKQDEVV YYDPCENPEE LKVIDCVVGL QDDKNLEVKE LRRQCQQLES KRGRICAKRA
481SLRSRKDQCK ENHRFRLQQA EESIRYSRQH HSIQMKRDKI KEEEQKKKEW INQERQKTLQ
541RLRSFKDKRL AQSVRNTSGS EPVAPNLPSD LSQQMCLPAS HAVSVIHPSS RKTRGVPLSE
601AGNVKSPKCQ NCHGNIPVQV FVPVGDQTHS KSSEELSLPP PPPPPPPPPP PPPPPPPPLR
661ALSSSSQAAT HQNLGFRAPV KDDQPRPLVC ESPAERPRDS LESFSCPGSM DEVLASLRHG
721RAPLRKVEVP AVRPPHASIN EHILAAIRQG VKLKKVHPDL GPNPSSKPTS NRRTSDLERS
781IKAALQRIKR VSADSEEDSD EQDPGQWDG
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

809 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.