Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
WEE1B / WEE2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G124148-100
Catalog Number: LS-G124148
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human WEE2 (NM_001105558), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- WEE1 homolog 2 (S. pombe) | WEE2 | Wee1-like protein kinase 1B | WEE1B | Wee1-like protein kinase 2 | Wee1B kinase
- Origin Species
- Human
- NCBI GenBank
- SK545D
- SwissProt
- P0C1S8
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 62.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDDKDIDKEL RQKLNFSYCE ETEIEGQKKV EESREASSQT PEKGEVQDSE AKGTPPWTPL61SNVHELDTSS EKDKESPDQI LRTPVSHPLK CPETPAQPDS RSKLLPSDSP STPKTMLSRL121VISPTGKLPS RGPKHLKLTP APLKDEMTSL ALVNINPFTP ESYKKLFLQS GGKRKIRGDL181EEAGPEEGKG GLPAKRCVLR ETNMASRYEK EFLEVEKIGV GEFGTVYKCI KRLDGCVYAI241KRSMKTFTEL SNENSALHEV YAHAVLGHHP HVVRYYSSWA EDDHMIIQNE YCNGGSLQAA301ISENTKSGNH FEEPKLKDIL LQISLGLNYI HNSSMVHLDI KPSNIFICHK MQSESSGVIE361EVENEADWFL SANVMYKIGD LGHATSINKP KVEEGDSRFL ANEILQEDYR HLPKADIFAL421GLTIAVAAGA ESLPTNGAAW HHIRKGNFPD VPQELSESFS SLLKNMIQPD AEQRPSAAAL481ARNTVLRPSL GKTEELQQQL NLEKFKTATL ERELREAQQA QSPQGYTHHG DTGVSGTHTG541SRSTKRLVGG KSARSSSFTS GEREPLHTRTRPLEQKLISEEDLAANDILDYKDDDDKV
567 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.