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WDTC1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

WDTC1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all WDTC1 antibodies →
$504.00each
Catalog Number: LS-G113526-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human WDTC1 (NM_015023), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DCAF9 | Adipose homolog | ADP | KIAA1037 | WDTC1
Origin Species
Human
NCBI GenBank
NM_015023
SwissProt
Q8N5D0

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
75.6 kda
Protein Type
Lysate
Amino Acid Sequence
1MAKVNITRDL IRRQIKERGA LSFERRYHVT DPFIRRLGLE AELQGHSGCV NCLEWNEKGD
61LLASGSDDQH TIVWDPLHHK KLLSMHTGHT ANIFSVKFLP HAGDRILITG AADSKVHVHD
121LTVKETIHMF GDHTNRVKRI ATAPMWPNTF WSAAEDGLIR QYDLRENSKH SEVLIDLTEY
181CGQLVEAKCL TVNPQDNNCL AVGASGPFVR LYDIRMIHNH RKSMKQSPSA GVHTFCDRQK
241PLPDGAAQYY VAGHLPVKLP DYNNRLRVLV ATYVTFSPNG TELLVNMGGE QVYLFDLTYK
301QRPYTFLLPR KCHSSGVQNG KMSTNGVSNG VSNGLHLHSN GFRLPESRGH VSPQVELPPY
361LERVKQQANE AFACQQWTQA IQLYSKAVQR APHNAMLYGN RAAAYMKRKW DGDHYDALRD
421CLKAISLNPC HLKAHFRLAR CLFELKYVAE ALECLDDFKG KFPEQAHSSA CDALGRDITA
481ALFSKNDGEE KKGPGGGAPV RLRSTSRKDS ISEDEMVLRE RSYDYQFRYC GHCNTTTDIK
541EANFFGSNAQ YIVSGSDDGS FFIWEKETTN LVRVLQGDES IVNCLQPHPS YCFLATSGID
601PVVRLWNPRP ESEDLTGRVV EDMEGASQAN QRRMNADPLE VMLLNMGYRI TGLSSGGAGA
661SDDEDSSEGQ VQCRPS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

676 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.