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WDR93 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

WDR93 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all WDR93 antibodies →
$504.00each
Catalog Number: LS-G111188-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human WDR93 (NM_020212), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
WD repeat domain 93 | WDR93
Origin Species
Human
NCBI GenBank
NM_020212
SwissProt
Q6P2C0

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
77.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSFPRGSQTQ KIKHPIGTRK GPLEVPPPTE KDWPKDDEQD HVLVDPDEEL DSLPQPYRMI
61NKLVNLLFDQ SWEIIEERNA LREAESSQIQ PTVYPPLGEI QLNKMPNCMA VSQDYVFIGG
121AKGFSIYNLY SAKQIYAWEK LKVDVTSIWA TDLGNEILIA PVDEMGIIRL FYFYKEGLYL
181VKAINEVDDT SKQTTCIKME ISQGGDFAAF LLQGAGDIWL DVYKLPKETW LKKLEHPQLT
241SNPKKKVRQP QLNSLGPISA DPLEMDANVS FKGDIKLSLP VYIMKIKPPK PVTGTTFKSP
301LEVFAKIKDC YGLGSGQNHF IKDSQWEQQA EIFNASYKKY LDREWEEEPL STATFYFLLP
361SCLFAMPPEV KGPSGMACVL GIHWTRSHNF FLYSLNRTLK DKADPEGVWP CAAPIAVSQL
421SCSSSYLVLA CEDGVLTLWD LAKGFPLGVA ALPQGCFCQS IHFLKYFSVH KGQNMYPEGQ
481VKSQMKCVVL CTDASLHLVE ASGTQGPTIS VLVERPVKHL DKTICAVAPV PALPGMVLIF
541SKNGSVCLMD VAKREIICAF APPGAFPLEV PWKPVFAVSP DHPCFLLRGD YSHETASTDD
601AGIQYSVFYF NFEACPLLEN ISKNCTIPQR DLDNMAFPQA LPLEKRCERF LQKSYRKLEK
661NPEKEEEHWA RLQRYSLSLQ RENFKK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

686 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.