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WDR91 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

WDR91 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all WDR91 antibodies →
$504.00each
Catalog Number: LS-G114141-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human WDR91 (NM_014149), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_014149

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
83.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAEAVERTDE LVREYLLFRG FTHTLRQLDA EIKADKEKGF RVDKIVDQLQ QLMQVYDLAA
61LRDYWSYLER RLFSRLEDIY RPTIHKLKTS LFRFYLVYTI QTNRNDKAQE FFAKQATELQ
121NQAEWKDWFV LPFLPSPDTN PTFATYFSRQ WADTFIVSLH NFLSVLFQCM PVPVILNFDA
181ECQRTNQVQE ENEVLRQKLF ALQAEIHRLK KEEQQPEEEE ALVQHKLPPY VSNMDRLGDS
241ELAMVCSQRN ASLSQSPRVG FLSSLLPQSK KSPSRLSPAQ GPPQPQSSAK KESFGGQGTK
301GKDPTSGAKD GKSLLSGLAT GESGWSQHRQ RRLQDHGKER KELFSTTTSQ CAEKKPEASG
361PEAEPCPELH TEPVEPLTRA SSAGPEGGGV RPEQPFIVLG QEEYGEHHSS IMHCRVDCSG
421RRVASLDVDG VIKVWSFNPI MQTKASSISK SPLLSLEWAT KRDRLLLLGS GVGTVRLYDT
481EAKKNLCEIN INDNMPRILS LACSPNGASF VCSAAAPSLT SQVDFSAPDI GSKGMNQVPG
541RLLLWDTKTM KQQLQFSLDP EPIAINCTAF NHNGNLLVTG AADGVIRLFD MQQHECAMSW
601RAHYGEVYSV EFSYDENTVY SIGEDGKFIQ WNIHKSGLKV SEYSLPSDAT GPFVLSGYSG
661YKQVQVPRGR LFAFDSEGNY MLTCSATGGV IYKLGGDEKV LESCLSLGGH RAPVVTVDWS
721TAMDCGTCLT ASMDGKIKLT TLLAHKA
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

747 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.