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WDR90 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

WDR90 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all WDR90 antibodies →
$725.00each
Catalog Number: LS-G106729-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human WDR90 (NM_145294), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_145294

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
187.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MARAWQHPFL NVFRHFRVDE WKRSAKQGDV AVVTDKTLKG AVYRIRGSVS AANYIQLPKS
61STQSLGLTGR YLYVLFRPLP SKHFVIHLDV SSKDNQVIRV SFSNLFKEFK STATWLQFPL
121VLEARTPQRD LVGLAPSGAR WTCLQLDLQD VLLVYLNRCY GHLKSIRLCA SLLVRNLYTS
181DLCFEPAISG AQWAKLPVTP MPREMAFPVP KGESWHDRYI HVRFPSESLK VPSKPIEKSC
241SPPEAVLLGP GPQPLPCPVA SSKPVRFSVS PVVQTPSPTA SGRAALAPRP FPEVSLSQER
301SDASNADGPG FHSLEPWAQL EASDIHTAAA GTHVLTHESA EVPVARTGSC EGFLPDPVLR
361LKGVIGFGGH GTRQALWTPD GAAVVYPCHA VIVVLLVDTG EQRFFLGHTD KVSALALDGS
421SSLLASAQAR APSVMRLWDF QTGRCLCLFR SPMHVVCSLS FSDSGALLCG VGKDHHGRTM
481VVAWGTGQVG LGGEVVVLAK AHTDFDVQAF RVTFFDETRM ASCGQGSVRL WRLRGGVLRS
541CPVDLGEHHA LQFTDLAFKQ ARDGCPEPSA AMLFVCSRSG HILEIDCQRM VVRHARRLLP
601TRTPGGPHPQ KQTFSSGPGI AISSLSVSPA MCAVGSEDGF LRLWPLDFSS VLLEAEHEGP
661VSSVCVSPDG LRVLSATSSG HLGFLDTLSR VYHMLARSHT APVLALAMEQ RRGQLATVSQ
721DRTVRIWDLA TLQQLYDFTS SEDAPCAVTF HPTRPTFFCG FSSGAVRSFS LEAAEVLVEH
781TCHRGAVTGL TATPDGRLLF SSCSQGSLAQ YSCADPQWHV LRVAADMVCP DAPASPSALA
841VSRDGRLLAF VGPSRCTVTV MGSASLDELL RVDIGTLDLA SSRLDSAMAV CFGPAALGHL
901LVSTSSNRVV VLDAVSGRII RELPGVHPEP CPSLTLSEDA RFLLIAAGRT IKVWDYATQA
961SPGPQVYIGH SEPVQAVAFS PDQQQVLSAG DAVFLWDVLA PTESDQSFPG APPACKTGPG
1021AGPLEDAASR ASELPRQQVP KPCQASPPRL GVCARPPEGG DGARDTRNSG APRTTYLASC
1081KAFTPARVSC SPHSAKGTCP PPASGGWLRL KAVVGYSGNG RANMVWRPDT GFFAYTCGRL
1141VVVEDLHSGA QQHWSGHSAE ISTLALSHSA QVLASASGRS STTAHCQIRV WDVSGGLCQH
1201LIFPHSTTVL ALAFSPDDRL LVTLGDHDGR TLALWGTATY DLVSSTRLPE PVHGVAFNPW
1261DAGELTCVGQ GTVTFWLLQQ RGADISLQVR REPVPEAVGA GELTSLCYGA PPLLYCGTSS
1321GQVCVWDTRA GRCFLSWEAD DGGIGLLLFS GSRLVSGSST GRLRLWAVGA VSELRCKGSG
1381ASSVFMEHEL VLDGAVVSAS FDDSVDMGVV GTTAGTLWFV SWAEGTSTRL ISGHRSKVNE
1441VVFSPGESHC ATCSEDGSVR VWALASMELV IQFQVLNQSC LCLAWSPPCC GRPEQQRLAA
1501GYGDGSLRIF SVSRTAMELK MHPHPVALTT VAFSTDGQTV LSGDKDGLVA VSHPCTGTTF
1561RVLSDHQGAP ISTICVTCKE CEDLGVEGTD LWLAASGDQR VSVWASDWLR NHCELVDWLS
1621FPMPATTETQ GHLPPSLAAF CPWDGALLMY VGPGVYKEVI IYNLCQKQVV EKIPLPFFAM
1681SLSLSPGTHL LAVGFAECML RLVDCAMGTA QDFAGHDNAV HLCRFTPSAR LLFTAARNEI
1741LVWEVPGL
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

1748 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.