LSBio
WDR89 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

WDR89 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all WDR89 antibodies →
$504.00each
Catalog Number: LS-G121866-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human WDR89 (NM_001008726), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C14orf150 | MSTP050 | WD repeat domain 89 | WDR89
Origin Species
Human
NCBI GenBank
N48542
SwissProt
Q96FK6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
43 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEKIEEQFAN LHIVKCSLGT KEPTYLLGID TSKTVQAGKE NLVAVLCSNG SIRIYDKERL
61NVLREFSGYP GLLNGVRFAN SCDSVYSACT DGTVKCWDAR VAREKPVQLF KGYPSNIFIS
121FDINCNDHII CAGTEKVDDD ALLVFWDARM NSQNLSTTKD SLGAYSETHS DDVTQVRFHP
181SNPNMVVSGS SDGLVNVFDI NIDNEEDALV TTCNSISSVS CIGWSGKGYK QIYCMTHDEG
241FYWWDLNHLD TDEPVTRLNI QDVREVVNMK EDALDYLIGG LYHEKTDTLH VIGGTNKGRI
301HLMNCSMSGL THVTSLQGGH AATVRSFCWN VQDDSLLTGG EDAQLLLWKP GAIEKTFTKK
361ESMKIASSVH QRVRVHSNDS YKRRKKQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

387 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.