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WDR78 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

WDR78 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all WDR78 antibodies →
$725.00each
Catalog Number: LS-G109766-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human WDR78 (NM_024763), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
RP11-342H21.1 | WD repeat domain 78 | WDR78
Origin Species
Human
NCBI GenBank
NM_024763
SwissProt
Q5VTH9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
94.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MTPGKHSGAS ARAANAGAWG YRDFRGGQKK GWWTTPQLVA TMPVSPAGSH KQQNFGLNNA
61TQPKKSISFF ATMKATSVKG YTGANQSRMA VSKTVLIPPE LKTVEKPNPN IKTTQVFDIN
121GTDVTPRPLY HPDPLTGTAK PSKLLTSQEG SLGSEFISSY SLYQNTINPS TLGQFTRSVL
181GSSTVSKSSV SASESIAEDL EEPSYKRERL TSFTDLQVIR AAPEKIVTKE DLEKNIEIIL
241TETETLRFFD LPTVMVSVES EEAEKVTQRN KNYEVLCRNR LGNDLYVERM MQTFNGAPKN
301KDVQCDKIIM EDKGIMSTAW DLYDSYNAME LVSLSVKQSV VESSSKANVL PKDQDQRLPG
361STTEKNSETS SLMDIENVIL AKIHEDEEDH SDAILKSDKF HQDLFFMERV LMENIFQPKL
421AAYRQLPVLK EPEPEEPEDV LESAKHEEVE EESKKEEEEE IHAEESTIPA NLERLWSFSC
481DLTKGLNVSS LAWNKTNPDL LAVGYGHFGF KEQKRGLACC WSIKNPMWPE RIYQSPYGVT
541AVDFSIGAPN LLAVGYHNGT IAIYNVRSNS NVPVLDSSES PQKHLGPVWQ LQWIEQDRGT
601TGDGKREILV SISADGRISK WVIRKGLDCY DLMRLKRTTA ASNKKGGEKE KKDEALISRQ
661APGMCFAFHP KDTNIYLAGT EEGHIHKCSC SYNEQYLDTY RGHKGPVYKV TWNPFCHDVF
721LSCSADWGVI IWQQENVKPS LSFYPATSVV YDVAWSPKSS YIFAAANENR VEIWDLHIST
781LDPLIVNTAN PGIKFTTILF AKQTDCLLVG DSDGQVSVYE LRNMPTVLET GRGDIMDTLL
841GSKSNQSA
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

848 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.