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WDR76 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

WDR76 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all WDR76 antibodies →
$504.00each
Catalog Number: LS-G109697-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human WDR76 (NM_024908), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CDW14 | WD repeat domain 76 | WDR76
Origin Species
Human
NCBI GenBank
NM_024908

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
69.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSRSGAAAEK ADSRQRPQMK VNEYKENQNI AYVSLRPAQT TVLIKTAKVY LAPFSLSNYQ
61LDQLMCPKSL SEKNSNNEVA CKKTKIKKTC RRIIPPKMKN TSSKAESTLQ NSSSAVHTES
121NKLQPKRTAD AMNLSVDVES SQDGDSDEDT TPALDFSGLS PYERKRLKNI SENADFFASL
181QLSESAARLR EMIEKRQPPK SKRKKPKREN GIGCRRSMRL LKVDPSGVSL PAAPTPPTLV
241ADETPLLPPG PLEMTSENQE DNNERFKGFL HTWAGMSKPS SKNTEKGLSS IKSYKANLNG
301MVISEDTVYK VTTGPIFSMA LHPSETRTLV AVGAKFGQVG LCDLTQQPKE DGVYVFHPHS
361QPVSCLYFSP ANPAHILSLS YDGTLRCGDF SRAIFEEVYR NERSSFSSFD FLAEDASTLI
421VGHWDGNMSL VDRRTPGTSY EKLTSSSMGK IRTVHVHPVH RQYFITAGLR DTHIYDARRL
481NSRRSQPLIS LTEHTKSIAS AYFSPLTGNR VVTTCADCNL RIFDSSCISS KIPLLTTIRH
541NTFTGRWLTR FQAMWDPKQE DCVIVGSMAH PRRVEIFHET GKRVHSFGGE YLVSVCSINA
601MHPTRYILAG GNSSGKIHVF MNEKSC
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

626 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.