LSBio
WDR75 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

WDR75 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all WDR75 antibodies →
$504.00each
Catalog Number: LS-G108991-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human WDR75 (NM_032168), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
NET16 | UTP17 | WDR75 | WD repeat domain 75
Origin Species
Human
NCBI GenBank
NM_032168
SwissProt
Q8IWA0

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
94.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MVEEENIRVV RCGGSELNFR RAVFSADSKY IFCVSGDFVK VYSTVTEECV HILHGHRNLV
61TGIQLNPNNH LQLYSCSLDG TIKLWDYIDG ILIKTFIVGC KLHALFTLAQ AEDSVFVIVN
121KEKPDIFQLV SVKLPKSSSQ EVEAKELSFV LDYINQSPKC IAFGNEGVYV AAVREFYLSV
181YFFKKKTTSR FTLSSSRNKK HAKNNFTCVA CHPTEDCIAS GHMDGKIRLW RNFYDDKKYT
241YTCLHWHHDM VMDLAFSVTG TSLLSGGRES VLVEWRDATE KNKEFLPRLG ATIEHISVSP
301AGDLFCTSHS DNKIIIIHRN LEASAVIQGL VKDRSIFTGL MIDPRTKALV LNGKPGHLQF
361YSLQSDKQLY NLDIIQQEYI NDYGLIQIEL TKAAFGCFGN WLATVEQRQE KETELELQMK
421LWMYNKKTQG FILNTKINMP HEDCITALCF CNAEKSEQPT LVTASKDGYF KVWILTDDSD
481IYKKAVGWTC DFVGSYHKYQ ATNCCFSEDG SLLAVSFEEI VTIWDSVTWE LKCTFCQRAG
541KIRHLCFGRL TCSKYLLGAT ENGILCCWNL LSCALEWNAK LNVRVMEPDP NSENIAAISQ
601SSVGSDLFVF KPSEPRPLYI QKGISREKVQ WGVFVPRDVP ESFTSEAYQW LNRSQFYFLT
661KSQSLLTFST KSPEEKLTPT SKQLLAEESL PTTPFYFILG KHRQQQDEKL NETLENELVQ
721LPLTENIPAI SELLHTPAHV LPSAAFLCSM FVNSLLLSKE TKSAKEIPED VDMEEEKESE
781DSDEENDFTE KVQDTSNTGL GEDIIHQLSK SEEKELRKFR KIDYSWIAAL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

830 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.