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WDR72 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

WDR72 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all WDR72 antibodies →
$504.00each
Catalog Number: LS-G104116-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human WDR72 (NM_182758), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_182758

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
123.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MRTSLQAVAL WGQKAPPHSI TAIMITDDQR TIVTGSQEGQ LCLWNLSHEL KISAKELLFG
61HSASVTCLAR ARDFSKQPYI VSAAENGEMC VWNVTNGQCV EKATLPYRHT AICYYHCSFR
121MTGEGWLLCC GEYQDVLIID AKTLAVVHSF RSSQFPDWIN CMCIVHSMRI QEDSLLVVSV
181AGELKVWDLS SSINSIQEKQ DVYEKESKFL ESLNCQTIRF CTYTERLLLV VFSKCWKVYD
241YCDFSLLLTE VSRNGQFFAG GEVIAAHRIL IWTEDGHSYI YQLLNSGLSK SIYPADGRVL
301KETIYLHLLC STSVQENKEQ SRPFVMGYMN ERKEPFYKVL FSGEVSGRIT LWHIPDVPVS
361KFDGSPREIP VTATWTLQDN FDKHDTMSQS IIDYFSGLKD GAGTAVVTSS EYIPSLDKLI
421CGCEDGTIII TQALNAAKAR LLEGGSLVKD SPPHKVLKGH HQSVTSLLYP HGLSSKLDQS
481WMLSGDLDSC VILWDIFTEE ILHKFFLEAG PVTSLLMSPE KFKLRGEQII CCVCGDHSVA
541LLHLEGKSCL LHARKHLFPV RMIKWHPVEN FLIVGCADDS VYIWEIETGT LERHETGERA
601RIILNCCDDS QLVKSVLPIA SETLKHKSIE QRSSSPYQLG PLPCPGLQVE SSCKVTDAKF
661CPRPFNVLPV KTKWSNVGFH ILLFDLENLV ELLLPTPLSD VDSSSSFYGG EVLRRAKSTV
721EKKTLTLRKS KTACGPLSAE ALAKPITESL AQGDNTIKFS EENDGIKRQK KMKISKKMQP
781KPSRKVDASL TIDTAKLFLS CLLPWGVDKD LDYLCIKHLN ILKLQGPISL GISLNEDNFS
841LMLPGWDLCN SGMIKDYSGV NLFSRKVLDL SDKYTATLPN QVGIPRGLEN NCDSLRESDT
901IVYLLSRLFL VNKLVNMPLE LACRVGSSFR MESIHNKMRG AGNDILNMSS FYSCLRNGKN
961ESHVPEADLS LLKLISCWRD QSVQVTEAIQ AVLLAEVQQH MKSLGKIPVN SQPVSMAENG
1021NCEMKQMLPK LEWTEELELQ CVRNTLPLQT PVSPVKHDSN SNSANFQDVE DMPDRCALEE
1081SESPGEPRHH SWIAKVCPCK VS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1102 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.