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WDR70 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

WDR70 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all WDR70 antibodies →
$504.00each
Catalog Number: LS-G112048-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human WDR70 (NM_018034), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
WDR70 | WD repeat domain 70
Origin Species
Human
NCBI GenBank
NM_018034
SwissProt
Q9NW82

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
73 kDa
Protein Type
Lysate
Amino Acid Sequence
1MERSGPSEVT GSDASGPDPQ LAVTMGFTGF GKKARTFDLE AMFEQTRRTA VERSRKTLEA
61REKEEEMNRE KELRRQNEDI EPTSSRSNVV RDCSKSSSRD TSSSESEQSS DSSDDELIGP
121PLPPKMVGKP VNFMEEDILG PLPPPLNEEE EEAEEEEEEE EEEENPVHKI PDSHEITLKH
181GTKTVSALGL DPSGARLVTG GYDYDVKFWD FAGMDASFKA FRSLQPCECH QIKSLQYSNT
241GDMILVVSGS SQAKVIDRDG FEVMECIKGD QYIVDMANTK GHTAMLHTGS WHPKIKGEFM
301TCSNDATVRT WEVENPKKQK SVFKPRTMQG KKVIPTTCTY SRDGNLIAAA CQNGSIQIWD
361RNLTVHPKFH YKQAHDSGTD TSCVTFSYDG NVLASRGGDD SLKLWDIRQF NKPLFSASGL
421PTMFPMTDCC FSPDDKLIVT GTSIQRGCGS GKLVFFERRT FQRVYEIDIT DASVVRCLWH
481PKLNQIMVGT GNGLAKVYYD PNKSQRGAKL CVVKTQRKAK QAETLTQDYI ITPHALPMFR
541EPRQRSTRKQ LEKDRLDPLK SHKPEPPVAG PGRGGRVGTH GGTLSSYIVK NIALDKTDDS
601NPREAILRHA KAAEDSPYWV SPAYSKTQPK TMFAQVESDD EEAKNEPEWK KRKI
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

654 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.