LSBio
WDR66 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

WDR66 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all WDR66 antibodies →
$504.00each
Catalog Number: LS-G106920-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human WDR66 (NM_144668), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
WDR66 | WD repeat domain 66
Origin Species
Human
SwissProt
Q8TBY9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
129.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSDAAEAPRE ATGENGETEM KEEEEPNPNY KEVEDPQQES KDDTIAWRES QEEERKTGEE
61EGEEEEKEEE GKEDKKIVME ETEEKAGEVQ EKEASGIQEE TTVEPQEVTA SMIRLETQIT
121DSQSITSGIF PKTQRGSKSK LSLQLEDAET DELLRDLSTQ IEFLDLDQIS PEEQQISSPE
181RQPSGELEEK TDRMPQDELG QERRDLEPEN REEGQERRVS DIQSKAGISR ESLVSSTTED
241ILFQKDKSTP VYPLTMTWSF GWNSSLPVYY IREERQRVLL YVCAHTAIIY NVFRNNQYHL
301QGHANIISCL CVSEDRRWIA TADKGPDCLV IIWDSFTGIP VHTIFDSCPE GNGIMAMAMT
361HDAKYLATIS DAEVQKVCIW KWTLAVETPA CTLELPTEYG VQNYVTFNPT NNKELVSNSK
421TRAIYYAWYE ERDTLAHSAP LLTEKTFNKL VGKFSQSIFH LNLTQILSAT MEGKLVVWDI
481HRPPSSASTF LGFPYIKPCK LVHLQKEGIT VLTTIDSYIV TGDIKGNIKF YDHTLSIVNW
541YSHLKLGAIR TLSFSKTPAT PPTEKSNYPP DCTLKGDLFV LRNFIIGTSD AAVYHLTTDG
601TKLEKLFVEP KDAICAISCH PYQPLIAIGS ICGMIKVWNY ENKQYLFSRV FEKGFGVQSL
661TYNPEGALLG AGFTEGTVYI LDAMSLENES PEPFKYSRTS VTHISFSHDS QYMATADRSF
721TVAVYMLVVR NGQRVWEYLA RLRSHRKSIR SLLFGVYLDS NEPRLLSLGT DRLLIEYDLL
781RSYKDHLEVL DIHHTDQGCY PTCMVWYPPL TRELFLLICN SGYKVKLFNA TTKMCRKTLL
841GPAYGSPIEQ TQVLPVRSMA ELQKRYLVFI NRDKLGLQIL PVDGNPHKTS AIVCHPNGVA
901GMAVSYDGCY AFTAGGHDRS VVQWKITLSV LEAAVSLGGE DLTPFYGLLS GGREGKFYRE
961LEDYFYYSQL RSQGIDTMET RKVSEHICLS ELPFVMRAIG FYPSEEKIDD IFNEIKFGEY
1021VDTGKLIDKI NLPDFLKVYL NHKPPFGNTM SGIHKSFEVL GYTNSKGKKA IRREDFLRLL
1081VTKGEHMTEE EMLDCFASLF GLNPEGWKSE PATCSVKGSE ICLEEELPDE ITAEIFATEI
1141LGLTISEDSG QDGQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1154 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.