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WDR63 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

WDR63 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all WDR63 antibodies →
$504.00each
Catalog Number: LS-G106748-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human WDR63 (NM_145172), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
RP11-507C22.2 | WD repeat domain 63 | NYD-SP29 | WDR63
Origin Species
Human
NCBI GenBank
AA405514
SwissProt
Q8IWG1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
102.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAPKQKKKTS RGKKRLKPVL AASEDMEPVN MESMGHPEIY PLVLTTKTQE IFNCRIDEDV
61TDEQPYKLIN KEDIFEDLRN RAAVSDFHPV KKIVQEYPGN ELLLVYDKDF KYGLNFYLIA
121TEEGKENYLN PPEVPEEQEE YKEHIPEDVY IYKPPVSKPW VSLGSEKEIE EESVTESTKQ
181ITYMISRKRS EFGAPIKFSD QNASSVKDAY IECTAYPDKN FTLKQLEKDV GMQVIPQIKD
241ISTQTKWTYP KNATTQYYPR EFSEEEKETL KQSKPLVDFL NNASISVEIA LQQNEIMNTF
301IDDWKYLAEE EGTFGDKTDT HLKEYQSFTD LHSPTEKMIT CVSWHPTIYG LIAVSVAVRL
361SFEDRVHFSG KLLLQPSLIL FWSFSDPIHP QLMLESPDDI FCFKFCPSDP NIIAGGCING
421QIVMWDITAH ADRIENIKAG GSRSKRATLK PMFLLEPESN KEAMYIRHCA VSSIENGHKK
481VITDIHWLSD TFEINRMGSV FENRSGICCQ LVTCSADCTI CFWDIRPQKP LTPQTTEKKK
541EESIEIPFDV PSTFLHLDLS WKPLTKVRLS KGETSLDHCP TKISLNEDHL LCKTQDKMLA
601QSKTEKAEEM NPYHNLESGM ANLLKPIDDF CTKFFVGTEE GEVIYTDWKM EKDPETGRLM
661SKKPVSHHTI HDGTVHTIQR SPFYNDIILT VGGWNVAIWK EGVMTGPLLQ SCCAPKRYTS
721GHWSLTRPGV FYIGREDGYI DIWDLLEKTH EPAQSQNICI TMITYIKPWI FSSKQQFIAT
781ADYYGTLHIL EIPWTLSRPS TNEMASVNHY FEREVKHLEY VEQRKKIREQ EKKEMELEMA
841KKKVKTYQKS KEQMQAELKM DYESYLELEK TVLINLGLIK VTEKGSYMEV M
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

891 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.