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WDR6 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

WDR6 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all WDR6 antibodies →
$725.00each
Catalog Number: LS-G129159-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human WDR6 (NM_018031), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
WD repeat-containing protein 6 | Wd-repeat protein 6 | WDR6 | WD repeat domain 6
Origin Species
Human
NCBI GenBank
AF099100
SwissProt
Q9NNW5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
124.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGSAARWEGS FSREFSSLLS RGCLSTSRID MDALEDYVWP RATSELILLP VTGLECVGDR
61LLAGEGPDVL VYSLDFGGHL RMIKRVQNLL GHYLIHGFRV RPEPNGDLDL EAMVAVFGSK
121GLRVVKISWG QGHFWELWRS GLWNMSDWIW DARWLEGNIA LALGHNSVVL YDPVVGCILQ
181EVPCTDRCTL SSACLIGDAW KELTIVAGAV SNQLLVWYPA TALADNKPVA PDRRISGHVG
241IIFSMSYLES KGLLATASED RSVRIWKVGD LRVPGGRVQN IGHCFGHSAR VWQVKLLENY
301LISAGEDCVC LVWSHEGEIL QAFRGHQGRG IRAIAAHERQ AWVITGGDDS GIRLWHLVGR
361GYRGLGVSAL CFKSRSRPGT LKAVTLAGSW RLLAVTDTGA LYLYDVEVKC WEQLLEDKHF
421QSYCLLEAAP GPEGFGLCAM ANGEGRVKVV PINTPTAAVD QTLFPGKVHS LSWALRGYEE
481LLLLASGPGG VVACLEISAA PSGKAIFVKE RCRYLLPPSK QRWHTCSAFL PPGDFLVCGD
541RRGSVLLFPS RPGLLKDPGV GGKARAGAGA PVVGSGSSGG GNAFTGLGPV STLPSLHGKQ
601GVTSVTCHGG YVYTTGRDGA YYQLFVRDGQ LQPVLRQKSC RGMNWLAGLR IVPDGSMVIL
661GFHANEFVVW NPRSHEKLHI VNCGGGHRSW AFSDTEAAMA FAYLKDGDVM LYRALGGCTR
721PHVILREGLH GREITCVKRV GTITLGPEYG VPSFMQPDDL EPGSEGPDLT DIVITCSEDT
781TVCVLALPTT TGSAHALTAV CNHISSVRAV AVWGIGTPGG PQDPQPGLTA HVVSAGGRAE
841MHCFSIMVTP DPSTPSRLAC HVMHLSSHRL DEYWDRQRNR HRMVKVDPET RYMSLAVCEL
901DQPGLGPLVA AACSDGAVRL FLLQDSGRIL QLLAETFHHK RCVLKVHSFT HEAPNQRRRL
961LLCSAATDGS LAFWDLTTML DHDSTVLEPP VDPGLPYRLG TPSLTLQAHS CGINSLHTLP
1021TREGHHLVAS GSEDGSLHVF VLAVEMLQLE EAVGEAGLVP QLRVLEEYSV PCAHAAHVTG
1081LKILSPSIMV SASIDQRLTF WRLGHGEPTF MNSTVFHVPD VADMDCWPVS PEFGHRCALG
1141GQGLEVYNWY D
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1151 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.