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WDR49 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

WDR49 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all WDR49 antibodies →
$504.00each
Catalog Number: LS-G104590-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human WDR49 (NM_178824), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
WD repeat domain 49 | WDR49
Origin Species
Human
SwissProt
Q8IV35

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
79.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAWREKSKKR LNMTSFNIAQ GIHAFDYHSR LNLIATAGIN NKVCLWNPYV VSKPVGVLWG
61HSASVIAVQF FVERKQLFSF SKDKVLRLWD IQHQLSIQRI ACSFPKSQDF RCLFHFDEAH
121GRLFISFNNQ LALLAMKSEA SKRVKSHEKA VTCVLYNSIL KQVISSDTGS TVSFWMIDTG
181QKIKQFTGCH GNAEISTMAL DANETRLLTG STDGTVKIWD FNGYCHHTLN VGQDGAVDIS
241QILILKKKIL VTGWERAITV FRPQNFNQFF IQPEEWKGGI QHHDDILCAA FLPPQTLVTG
301SYDGEIVLWN NSTENAHHVL HPDYQRLLKS KLDTKPQKLL SAGRSQPSHP MADHSTTGVR
361NFEIDTEGKN AVMRLCFLKA RKNTAVTGGA NLVSCGGSGY VRFWDIYKKQ LLAEFLAHSG
421VGSIIMSTDK MNRYLTTGDL DGWLKIWNIE EYCLNSSKNK ITKAPTLIRS FQPHEDRISS
481LEMCEPGGQL LIISSSADCS ICVTGVCNAP VWIFGQAKHW HIENCLFLPK RDTNLVESEI
541QKEISLFSKE ESCLDPTEHS LLNKKNKDDS TYNVRPSEDI NLDIKYKERS TCMKETQKPY
601YGEVIKKSFS TFRSLNIGAL EELPEVNKPA FLLDPEKYFR KEPEEERPQI LEAPSLFKTL
661KAVFDEKNLF PKEILHHERK AKQLCQEKSC EVKKNKK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

697 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.